FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST8SIA5



General Information
Gene Symbol:   
ST8SIA5
Protein name:   
Alpha-2,8-sialyltransferase 8E
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.8
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• ST8Sia-I, ST8Sia-III, and ST8Sia-V show broad activities, but distinct specificity, toward most gangliosides. ST8Sia-V shows preference for GD1a and GT1b (KM~70-80 mM), higher for GD3 and highest for GM1b and α2-3sialylparaglobosides. ST8Sia-3 is better for GD3 and α2-3sialylparaglobosides, and ST8Gal-I prefers GM3 (KM=30mM).
• Mainly expressed in the brain and the expression increased with aging.

Related Pathway
KEGG ORTHOLOGY:   
K03369
KEGG Pathway:   
ko00604  ko01100 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
O15466 
NCBI GeneID:   
29906
GlyMap GeneID:   
29906
DNA aRefSeq:   
NM_001307986.1 
Protein RefSeq:   
NP_001294915.1 
BRENDA:   
Kinetic info.
OMIM number:   
607162
GeneCards database:   
ST8SIA5
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-3)Gal(b1-3)GalNAc(b1-4) or NeuAc(a2-8)NeuAc(a2-3)Gal(b1-3)Glc(b1-?)Cer
Product:  
     NeuAc(a2-8)NeuAc(a2-3)Gal(b1-3)GalNAc(b1-4) or NeuAc(a2-8)NeuAc(a2-8)NeuAc(a2-3)Gal(b1-3)Glc(b1-?)Cer
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-671-5p 805-812 -0.625 99.0 -0.625 99.0 noncon
hsa-miR-3615 627-634 -0.567 98.0 -0.567 98.0 noncon
hsa-miR-3615 336-343 -0.554 98.0 -0.554 98.0 noncon
hsa-miR-363-5p 318-325 -0.543 99.0 -0.543 99.0 noncon
hsa-miR-6727-5p 69-76 -0.519 96.0 -0.519 97.0 noncon
hsa-miR-6745 318-325 -0.514 99.0 -0.514 99.0 noncon
hsa-miR-4669 712-719 -0.463 99.0 -0.463 99.0 noncon
hsa-miR-6861-3p 572-579 -0.453 98.0 -0.453 98.0 noncon
hsa-miR-937-5p 825-832 -0.401 99.0 -0.401 99.0 noncon
hsa-miR-6778-3p 152-159 -0.381 98.0 -0.381 98.0 noncon
hsa-miR-6797-5p 113-120 -0.37 98.0 -0.37 98.0 noncon
hsa-miR-6815-3p 524-531 -0.367 97.0 -0.367 97.0 noncon
hsa-miR-4639-5p 839-846 -0.363 98.0 -0.363 99.0 noncon
hsa-miR-1249-5p 113-120 -0.362 97.0 -0.362 98.0 noncon
hsa-miR-4687-3p 587-594 -0.357 97.0 -0.357 98.0 noncon
hsa-miR-6777-3p 403-410 -0.355 97.0 -0.355 98.0 noncon
hsa-miR-301b-3p 796-803 -0.355 98.0 -0.355 98.0 con
hsa-miR-301a-3p 796-803 -0.355 98.0 -0.355 98.0 con
hsa-miR-7-5p 862-868 -0.353 98.0 -0.353 98.0 noncon
hsa-miR-454-3p 796-803 -0.35 98.0 -0.35 98.0 con
hsa-miR-3666 796-803 -0.347 98.0 -0.347 98.0 con
hsa-miR-130b-3p 796-803 -0.347 98.0 -0.347 98.0 con
hsa-miR-4508 68-75 -0.344 96.0 -0.344 97.0 noncon
hsa-miR-671-5p 849-855 -0.344 97.0 -0.344 98.0 noncon
hsa-miR-6874-5p 876-882 -0.342 96.0 -0.342 97.0 noncon
hsa-miR-130a-3p 796-803 -0.339 97.0 -0.339 98.0 con
hsa-miR-6079 806-813 -0.339 98.0 -0.339 98.0 noncon
hsa-miR-4295 796-803 -0.339 98.0 -0.339 98.0 con
hsa-miR-105-3p 333-339 -0.338 92.0 -0.338 93.0 noncon
hsa-miR-601 541-547 -0.334 93.0 -0.334 95.0 noncon
hsa-miR-653-3p 550-557 -0.329 98.0 -0.329 98.0 noncon
hsa-miR-218-5p 309-315 -0.327 96.0 -0.327 97.0 con
hsa-miR-6752-3p 156-162 -0.321 95.0 -0.321 96.0 noncon
hsa-miR-3160-3p 874-880 -0.32 97.0 -0.32 98.0 noncon
hsa-miR-1182 380-387 -0.314 97.0 -0.314 98.0 noncon
hsa-miR-296-5p 283-289 -0.314 91.0 -0.314 93.0 noncon
hsa-miR-943 439-446 -0.314 97.0 -0.314 98.0 noncon
hsa-miR-4633-3p 876-882 -0.31 97.0 -0.31 98.0 noncon
hsa-miR-324-3p 172-179 -0.309 96.0 -0.309 97.0 noncon
hsa-miR-3914 465-471 -0.308 97.0 -0.308 98.0 noncon
hsa-miR-6842-3p 788-794 -0.301 96.0 -0.301 97.0 noncon
hsa-miR-3162-3p 148-155 -0.298 91.0 -0.298 94.0 noncon
hsa-miR-6515-5p 114-120 -0.296 95.0 -0.296 96.0 noncon
hsa-miR-1913 172-179 -0.291 96.0 -0.291 97.0 noncon
hsa-miR-4421 410-417 -0.285 95.0 -0.285 96.0 noncon
hsa-miR-4802-5p 115-121 -0.282 96.0 -0.282 97.0 noncon
hsa-miR-6868-3p 770-777 -0.282 96.0 -0.282 97.0 noncon
hsa-miR-663b 264-271 -0.28 95.0 -0.28 96.0 noncon
hsa-miR-6732-3p 158-165 -0.28 96.0 -0.28 97.0 noncon
hsa-miR-4694-3p 879-886 -0.274 98.0 -0.274 98.0 noncon
hsa-miR-6500-5p 876-882 -0.274 96.0 -0.274 97.0 noncon
hsa-miR-6744-5p 331-337 -0.27 94.0 -0.27 96.0 noncon
hsa-miR-644a 596-602 -0.267 93.0 -0.267 95.0 noncon
hsa-miR-5699-3p 410-417 -0.267 94.0 -0.267 96.0 noncon
hsa-miR-4330 677-684 -0.265 97.0 -0.265 98.0 noncon
hsa-miR-8081 826-832 -0.265 95.0 -0.265 96.0 noncon
hsa-miR-4308 576-583 -0.263 96.0 -0.263 97.0 noncon
hsa-miR-1293 319-325 -0.263 95.0 -0.263 96.0 noncon
hsa-miR-4701-3p 320-326 -0.262 95.0 -0.262 96.0 noncon
hsa-miR-3921 514-520 -0.259 97.0 -0.259 97.0 noncon
hsa-miR-6837-3p 552-559 -0.258 95.0 -0.258 96.0 noncon
hsa-miR-1245b-3p 675-682 -0.257 97.0 -0.257 97.0 noncon
hsa-miR-4483 319-325 -0.257 97.0 -0.257 98.0 noncon
hsa-miR-4712-3p 916-922 -0.252 96.0 -0.19 95.0 noncon
hsa-miR-3189-3p 640-646 -0.25 94.0 -0.25 95.0 noncon
hsa-miR-4661-3p 139-145 -0.25 92.0 -0.25 94.0 noncon