EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 

Pathway Map

ST8SIA2



General Information
Gene Symbol:   
ST8SIA2
Protein name:   
Alpha-2,8-sialyltransferase 8B
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   
+
+ CMP
Reaction (IUBMB):   
No specified reaction.

About

• ST8SIA2 is characterized by the ability to synthesize polysialic acid (polySia) structures and can therefore be sub-classified as a polysialyltransferase. Sia polymers can have upto 400 Sia units.
• ST8SIA2 transfers Sia residue from CMP-Sia to Siaα2,3Gal- and Siaα2,6Gal/GalNAc on N-linked and/or O-linked glycans.
• Its function is similar to another polySia enzyme ST8Sia-4, though both proteins are expressed in different spatiotemporal patterns.
• Restricted activity has been shown mostly to brain proteins especially NCAM and SynCAM-1 in the IgG domain, though it can also act on other proteins with lower activity. Thus, there is a protein dependence for enzyme activity.
• PolySia imparts an anti-adhesive effect due to the bulky, polyanionic structure.
• It is implicated in psychiatric disorders and cancer.
• Endo-N-acylneuraminidase (Endo-N) specifically cleaves α2,8-linked polySia.
• ST8Sia-2 plus -4 DKO mice show severe phenotypes, such as postnatal growth retardation, precocious death, a high incidence of hydrocephalus, and agenesis or hypoplasia of major brain fiber tracts.
• While poly-Sia is not common to normal tissue, a number of cancers express polySia including neuroblastomas, Wilms tumors, medulloblastomas, pheochromocytomas, medullary thyroid carcinomas, non-small-cell lung carcinomas (NSCL), pituitary adenomas and breast cancer.


Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
BRENDA:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type)
Compartment:  
     
Substrate:  
     NeuAc(a2-?)
Product:  
     NeuAc(a2-8)NeuAc(a2-?)

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data

miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-596 92-99 -0.408 99.0 -0.408 99.0 noncon
hsa-miR-5090 2010-2017 -0.402 95.0 -0.402 96.0 noncon
hsa-miR-483-3p.1 742-749 -0.395 98.0 -0.395 99.0 noncon
hsa-miR-6775-5p 2010-2017 -0.389 94.0 -0.389 95.0 noncon
hsa-miR-541-5p 4335-4342 -0.376 99.0 -0.204 96.0 noncon
hsa-miR-342-5p 2153-2160 -0.369 97.0 -0.369 97.0 noncon
hsa-miR-6852-5p 2329-2336 -0.356 98.0 -0.356 98.0 noncon
hsa-miR-510-5p 170-177 -0.347 98.0 -0.347 98.0 noncon
hsa-miR-4664-5p 2153-2160 -0.327 96.0 -0.327 96.0 noncon
hsa-miR-1227-5p 3913-3919 -0.31 96.0 -0.31 97.0 noncon
hsa-miR-503-3p 1388-1395 -0.306 95.0 -0.306 97.0 noncon
hsa-miR-4446-3p 1861-1868 -0.304 96.0 -0.304 97.0 noncon
hsa-miR-4473 3706-3713 -0.301 91.0 -0.301 94.0 noncon
hsa-miR-4655-3p 942-949 -0.301 92.0 -0.301 94.0 noncon
hsa-miR-6721-5p 2475-2482 -0.3 94.0 -0.3 95.0 noncon
hsa-miR-5001-5p 1753-1760 -0.298 92.0 -0.298 94.0 noncon
hsa-miR-4498 1753-1760 -0.288 92.0 -0.288 93.0 noncon
hsa-miR-17-5p 4137-4144 -0.285 97.0 -0.285 98.0 con
hsa-miR-93-5p 4137-4144 -0.285 98.0 -0.285 98.0 con
hsa-miR-20b-5p 4137-4144 -0.285 97.0 -0.285 98.0 con
hsa-miR-124-3p.1 3998-4004 -0.283 94.0 -0.283 96.0 con
hsa-miR-106a-5p 4137-4144 -0.282 97.0 -0.282 98.0 con
hsa-miR-4292 1431-1438 -0.276 95.0 -0.276 96.0 noncon
hsa-miR-1247-3p 2611-2618 -0.275 91.0 -0.275 93.0 noncon
hsa-miR-519d-3p 4137-4144 -0.269 97.0 -0.269 98.0 con
hsa-miR-6791-5p 1431-1438 -0.268 94.0 -0.268 96.0 noncon
hsa-miR-5580-5p 1498-1504 -0.268 92.0 -0.268 94.0 noncon
hsa-miR-6782-5p 3263-3269 -0.267 90.0 -0.267 92.0 noncon
hsa-miR-1587 1754-1761 -0.266 94.0 -0.266 95.0 noncon
hsa-miR-106b-5p 4137-4144 -0.264 97.0 -0.264 98.0 con
hsa-miR-6744-5p 1017-1023 -0.264 94.0 -0.264 96.0 noncon
hsa-miR-20a-5p 4137-4144 -0.264 97.0 -0.264 98.0 con
hsa-miR-605-3p 3404-3411 -0.264 93.0 -0.264 95.0 noncon
hsa-miR-4474-3p 977-984 -0.261 93.0 -0.261 95.0 noncon
hsa-miR-6864-5p 4046-4053 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-7515 4045-4052 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-1227-3p 71-78 -0.259 91.0 -0.259 95.0 noncon
hsa-miR-3675-5p 3914-3920 -0.251 91.0 -0.251 94.0 noncon
hsa-miR-5587-5p 128-134 -0.251 94.0 -0.251 95.0 noncon
hsa-miR-4283 1064-1071 -0.25 90.0 -0.25 92.0 noncon