EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 

Pathway Map

POFUT2



General Information
Gene Symbol:   
POFUT2
Protein name:   
GDP-fucose protein O-fucosyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   
+ Ser|Thr
+ GDP
Reaction (IUBMB):   
No specified reaction.

About

• ER resident protein-O-fucosyltransferases [POFUT1/FUT12 and POFUT2/FUT13] that attach alpha-Fuc to hydroxyl of Ser/Thr at specific consensus sequences of epidermal growth factor (EGF)-like repeats (added by POFUT1) and thrombospondin type 1 repeat (TSR, added by POFUT2).
• Putative consensus sequences for O-fucosylation in EGF repeats [C2-X-X-X-X-(S/T)-C3] and TSRs [C2-X-X-(S/T)-C3-X-X-G] have been defined, where Cx refers to the position of one of the conserved cysteines within the domain and S/T is the site of modification.
• Thus, these enzymes may fucosylate ~100 EGF repeat structures (by POFUT1) and ~30 TSRs (by POFUT2). EGF repeats of proteins bearing O-Fuc include tissue-type plasminogen activator, Factor VII, Factor IX, Notch, Delta, Serrate, Jagged, and Cripto. Examples of TSR1 modified proteins include members of the disintegrin and metalloprotease domain with thrombospondin type 1 repeats family (e.g., ADAMTS 5 and 13) and ADAMTS-like family (e.g., ADAMTSL1; Wang et al. 2007).
• Mice lacking POFUT1 are embryonically lethal, and those without POFUT2 die at E7.5. Thus, both POFUT1 and POFUT2 play essential roles in development.
• POFUT1 plays a key role in regulating Notch dependent signaling.
• The O-fucose on Notch EGF repeats are extended by the Fringe family of β3-GlcNAcTs. They can be further extended to create Siaα2-3/6Galβ1-4GlcNAcβ1-3Fucα-O-Ser/Thr.
• O-Fucose on TSRs is typically found extended to the disaccharide Glcβ1-3Fucα-O-Ser/Thr This extension elongation is due to a β3-glucosyltransferase. Mutations in the β3-glucosyltransferase result in the human developmental disorder Peters plus syndrome. Both O-fucosylated and C-mannosylation occur on TSR1/thrombospondin-1.


Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
BRENDA:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     ER
Substrate:  
     Ser|Thr
Product:  
     Fuc(a1-?)Ser|Thr

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 200 180 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000331343 ENST00000334538 ENST00000349485 ENST00000615172 IKZF1 USF1 MAX ARID1B TCF12 E2F6 NR2C2 STAT5A WT1 GATA2 BHLHE40 ZNF263 MYC ZNF264 NFE2 SP1 EGR3 EGR2 MYCN MYCN KMT2A NFE2 YY1


miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4488 749-756 -0.433 98.0 -0.433 98.0 noncon
hsa-miR-1237-5p 749-756 -0.428 98.0 -0.428 98.0 noncon
hsa-miR-4697-5p 749-756 -0.425 98.0 -0.425 98.0 noncon
hsa-miR-3196 786-793 -0.415 90.0 -0.415 92.0 noncon
hsa-miR-6815-5p 333-339 -0.38 98.0 -0.38 98.0 noncon
hsa-miR-6865-5p 333-339 -0.38 98.0 -0.38 98.0 noncon
hsa-miR-6761-3p 2167-2174 -0.369 91.0 -0.002 13.0 noncon
hsa-miR-4667-3p 1266-1273 -0.356 98.0 -0.356 98.0 noncon
hsa-miR-3131 1778-1784 -0.343 92.0 -0.002 12.0 noncon
hsa-miR-3166 461-468 -0.341 95.0 -0.341 97.0 noncon
hsa-miR-4512 621-628 -0.338 97.0 -0.338 98.0 noncon
hsa-miR-5008-5p 479-486 -0.337 96.0 -0.337 97.0 noncon
hsa-miR-219b-5p 1309-1316 -0.33 95.0 -0.33 96.0 noncon
hsa-miR-181a-2-3p 4728-4735 -0.325 98.0 -0.002 35.0 noncon
hsa-miR-4749-3p 1020-1027 -0.307 94.0 -0.307 95.0 noncon
hsa-miR-1269b 3237-3244 -0.305 95.0 -0.002 27.0 noncon
hsa-miR-1269a 3237-3244 -0.305 95.0 -0.002 28.0 noncon
hsa-miR-6852-3p 1255-1262 -0.299 94.0 -0.299 95.0 noncon
hsa-miR-124-3p.1 1443-1449 -0.297 95.0 -0.297 96.0 noncon
hsa-miR-3909 1255-1262 -0.291 93.0 -0.291 95.0 noncon
hsa-miR-611 1778-1784 -0.281 92.0 -0.001 16.0 noncon
hsa-miR-3591-3p 1374-1381 -0.278 96.0 -0.278 97.0 noncon
hsa-miR-5088-3p 4747-4754 -0.278 96.0 -0.001 31.0 noncon
hsa-miR-1909-5p 676-683 -0.277 93.0 -0.277 95.0 noncon
hsa-miR-5587-5p 1501-1507 -0.275 95.0 -0.275 96.0 noncon
hsa-miR-4326 1223-1230 -0.273 94.0 -0.273 96.0 noncon
hsa-miR-4660 948-954 -0.272 94.0 -0.272 96.0 noncon
hsa-miR-21-3p 1374-1381 -0.265 95.0 -0.265 97.0 noncon
hsa-miR-6764-5p 424-431 -0.263 97.0 -0.263 98.0 noncon
hsa-miR-1178-5p 1347-1353 -0.262 92.0 -0.262 94.0 noncon
hsa-miR-6810-3p 4438-4444 -0.262 92.0 -0.001 34.0 noncon
hsa-miR-504-5p.1 4662-4669 -0.261 97.0 -0.001 39.0 noncon
hsa-miR-615-5p 3027-3034 -0.255 91.0 -0.001 24.0 noncon
hsa-miR-7160-5p 4209-4216 -0.255 96.0 -0.001 33.0 noncon
hsa-miR-1260a 1922-1929 -0.254 92.0 -0.001 31.0 noncon
hsa-miR-1260b 1922-1929 -0.254 92.0 -0.001 30.0 noncon
hsa-miR-4430 329-336 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-660-3p 2154-2161 -0.251 95.0 -0.001 29.0 noncon