EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
PAPSS1



General Information
Gene Symbol:   
PAPSS1
Protein name:   
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.7.7.4
Tissues:   
all, Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   

ATP → PAPS
Reaction (IUBMB):   
No specified reaction.
About

• PAPSS1/2 have two functions of phosphorylation of ADP and sulfation of ATP. These are crucial for the formation of PAPS, which is the source for sulfates in glycan modifications.
• PAPSS2 deficiency has been shown to cause physical deformities later in life.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-174362
Related Database
Organism:   
Homo sapiens
Uniprot:   
O43252 
NCBI GeneID:   
9061
GlyMap GeneID:   
9061
DNA aRefSeq:   
NM_005443.4 
Protein RefSeq:   
NP_005434.4 
BRENDA:   
Kinetic info.
OMIM number:   
603262
GeneCards database:   
PAPSS1
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     ATP
Product:  
     PAPS
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-8065 223-230 -0.569 99.0 -0.569 99.0 noncon
hsa-miR-7107-3p 110-117 -0.513 99.0 -0.513 99.0 noncon
hsa-miR-6753-3p 110-117 -0.513 99.0 -0.513 99.0 noncon
hsa-miR-3923 34-41 -0.5 99.0 -0.5 99.0 noncon
hsa-miR-4302 178-185 -0.414 99.0 -0.414 99.0 noncon
hsa-miR-4786-3p 574-580 -0.403 98.0 -0.271 96.0 noncon
hsa-miR-8066 321-328 -0.4 99.0 -0.4 99.0 noncon
hsa-miR-4672 527-534 -0.388 99.0 -0.297 98.0 noncon
hsa-miR-6880-5p 17-23 -0.354 96.0 -0.354 97.0 noncon
hsa-miR-6783-5p 342-348 -0.342 98.0 -0.342 98.0 noncon
hsa-miR-4480 379-385 -0.329 97.0 -0.329 98.0 noncon
hsa-miR-601 385-391 -0.328 93.0 -0.328 95.0 noncon
hsa-miR-133a-3p.1 49-55 -0.321 92.0 -0.321 94.0 con
hsa-miR-1287-3p 383-389 -0.317 94.0 -0.317 96.0 noncon
hsa-miR-3714 145-152 -0.302 99.0 -0.302 99.0 noncon
hsa-miR-451b 331-337 -0.3 98.0 -0.3 98.0 noncon
hsa-miR-135b-3p 225-231 -0.284 90.0 -0.284 93.0 noncon
hsa-miR-6501-3p 535-541 -0.279 97.0 -0.209 96.0 noncon
hsa-miR-8061 506-513 -0.274 97.0 -0.212 96.0 noncon
hsa-miR-4775 561-568 -0.273 99.0 -0.186 99.0 noncon
hsa-miR-3668 438-444 -0.271 95.0 -0.209 93.0 noncon
hsa-miR-5692b 255-262 -0.269 99.0 -0.269 99.0 noncon
hsa-miR-5692c 255-262 -0.269 99.0 -0.269 99.0 noncon
hsa-miR-4760-5p 506-513 -0.269 97.0 -0.208 96.0 noncon
hsa-miR-592 26-32 -0.268 96.0 -0.268 97.0 noncon
hsa-miR-198 90-97 -0.266 96.0 -0.266 97.0 noncon
hsa-miR-3936 344-350 -0.265 95.0 -0.265 96.0 noncon
hsa-miR-628-3p 33-39 -0.264 94.0 -0.264 96.0 noncon
hsa-miR-4461 555-561 -0.261 96.0 -0.196 95.0 noncon
hsa-miR-1207-3p 495-501 -0.256 96.0 -0.198 95.0 noncon