GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MGAT1



General Information
Gene Symbol:   
MGAT1
Protein name:   
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT13
E.C. No:   
2.4.1.101
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• MGAT1 or GnT1 catalyzes the transfer of β1,2-linked GlcNAc from UDP-GlcNAc to the terminal α1,3-linked Man in Man5GlcNAc2Asn to initiate the synthesis of hybrid and complex N-glycans. This results in a substrate for a-mannosidase II (MAN2A1).
• If MA2A1 does not act then a hybrid structure may be formed by the addition of Gal & sialic acid to the GlcNAc.
• Mice with a null mutation in the Mgat1 gene die at E9.5.

Related Pathway
KEGG ORTHOLOGY:   
K00726
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-964739
Related Database
Organism:   
Homo sapiens
Uniprot:   
P26572 
NCBI GeneID:   
4245
GlyMap GeneID:   
4245
DNA aRefSeq:   
NM_001114617.1 
Protein RefSeq:   
NP_001108089.1 
BRENDA:   
Kinetic info.
OMIM number:   
160995
GeneCards database:   
MGAT1
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Man(a1-3)...Man(b1-4)
Product:  
     GlcNAc(b1-2)Man(a1-3)...Man(b1-4)
Constraints:  
     nMan(a1-?)=4
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    4.50e-04    (pmol/cell • hr)       KM:    2.60e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4701-5p 452-459 -0.594 99.0 -0.594 99.0 noncon
hsa-miR-662 4223-4230 -0.589 99.0 -0.004 18.0 noncon
hsa-miR-588 452-459 -0.578 99.0 -0.578 99.0 noncon
hsa-miR-4313 615-622 -0.518 98.0 -0.518 99.0 noncon
hsa-miR-6752-3p 1128-1135 -0.454 98.0 -0.006 40.0 noncon
hsa-miR-3137 1047-1054 -0.45 99.0 -0.45 99.0 noncon
hsa-miR-7160-3p 4365-4372 -0.449 98.0 -0.003 33.0 noncon
hsa-miR-1237-5p 3785-3792 -0.447 98.0 -0.006 22.0 noncon
hsa-miR-4488 3785-3792 -0.444 98.0 -0.006 20.0 noncon
hsa-miR-4697-5p 3785-3792 -0.444 98.0 -0.006 20.0 noncon
hsa-miR-4726-5p 908-915 -0.407 96.0 -0.407 97.0 noncon
hsa-miR-6834-3p 441-448 -0.399 98.0 -0.399 98.0 noncon
hsa-miR-6784-5p 1298-1305 -0.397 95.0 -0.006 25.0 noncon
hsa-miR-4687-5p 1169-1176 -0.388 98.0 -0.006 48.0 noncon
hsa-miR-874-5p 265-272 -0.378 96.0 -0.378 97.0 noncon
hsa-miR-4763-5p 1124-1131 -0.374 93.0 -0.005 31.0 noncon
hsa-miR-4640-5p 908-915 -0.373 96.0 -0.373 97.0 noncon
hsa-miR-4308 1233-1239 -0.371 98.0 -0.005 33.0 noncon
hsa-miR-4258 3297-3304 -0.351 96.0 -0.005 20.0 noncon
hsa-miR-501-3p 99-105 -0.345 97.0 -0.345 98.0 noncon
hsa-miR-502-3p 99-105 -0.345 97.0 -0.345 98.0 noncon
hsa-miR-4308 3880-3886 -0.331 98.0 -0.005 33.0 noncon
hsa-miR-665 4375-4381 -0.328 98.0 -0.002 35.0 noncon
hsa-miR-6756-3p 437-444 -0.327 95.0 -0.327 96.0 noncon
hsa-miR-6749-3p 1184-1191 -0.324 95.0 -0.005 38.0 noncon
hsa-miR-7114-3p 507-514 -0.321 95.0 -0.321 97.0 noncon
hsa-miR-939-5p 402-408 -0.32 96.0 -0.32 96.0 noncon
hsa-miR-4767 933-939 -0.319 90.0 -0.319 92.0 noncon
hsa-miR-1911-3p 4569-4575 -0.318 96.0 -0.002 31.0 noncon
hsa-miR-342-5p 3958-3964 -0.314 95.0 -0.005 31.0 noncon
hsa-miR-665 296-303 -0.313 97.0 -0.313 98.0 noncon
hsa-miR-4707-5p 1511-1518 -0.312 93.0 -0.005 26.0 noncon
hsa-miR-3127-3p 437-444 -0.311 95.0 -0.311 96.0 noncon
hsa-miR-500a-3p 98-104 -0.31 96.0 -0.31 97.0 noncon
hsa-miR-2116-3p 431-438 -0.309 98.0 -0.309 99.0 noncon
hsa-miR-1254 3224-3230 -0.302 98.0 -0.004 30.0 noncon
hsa-miR-1343-5p 402-408 -0.298 95.0 -0.298 96.0 noncon
hsa-miR-4713-5p 432-439 -0.285 96.0 -0.285 97.0 noncon
hsa-miR-4742-5p 1013-1020 -0.28 97.0 -0.28 98.0 noncon
hsa-miR-4664-5p 3958-3964 -0.28 94.0 -0.004 33.0 noncon
hsa-miR-4270 352-359 -0.28 96.0 -0.28 97.0 noncon
hsa-miR-6754-5p 352-359 -0.277 96.0 -0.277 97.0 noncon
hsa-miR-6867-3p 1147-1154 -0.275 95.0 -0.004 36.0 noncon
hsa-miR-26b-3p 4189-4196 -0.272 95.0 -0.004 37.0 noncon
hsa-miR-1224-3p 47-54 -0.272 96.0 -0.272 97.0 noncon
hsa-miR-297 4175-4182 -0.27 95.0 -0.004 35.0 noncon
hsa-miR-6746-5p 165-171 -0.268 90.0 -0.268 93.0 noncon
hsa-miR-4441 352-359 -0.266 96.0 -0.266 97.0 noncon
hsa-miR-3116 3224-3230 -0.265 97.0 -0.004 32.0 noncon
hsa-miR-1291 489-495 -0.264 91.0 -0.264 93.0 noncon
hsa-miR-6892-3p 718-725 -0.258 93.0 -0.258 95.0 noncon
hsa-miR-6809-5p 27-34 -0.257 92.0 -0.257 94.0 noncon
hsa-miR-4713-5p 1148-1154 -0.256 94.0 -0.004 24.0 noncon
hsa-miR-3157-5p 1073-1079 -0.252 95.0 -0.252 96.0 noncon
hsa-miR-6858-3p 787-793 -0.251 96.0 -0.251 97.0 noncon