GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MAN1A2



General Information
Gene Symbol:   
MAN1A2
Protein name:   
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB
Enzyme Class:   
Glycosidase
CAZy Family:   
GH47
E.C. No:   
3.2.1.113
Organs:   
Reaction (SNFG):   
MAN1A2
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Cleavage of four α1,2-mannose (α1,2-Man) residues from Man9GlcNAc2-Asn.
• ER resident Man1B1 first cleaves the single α1,2Man residue from the B branch of the Man9GlcNAc2-Asn.
• This intermediate is then rapidly cleaved in the Golgi by the concerted action of Golgi resident α-mannosidases IA, IB, and IC depending on the relative expression levels in specific cell types.
• Cleavage of Man by these enzymes is followed by the addition of GlcNAc by MGAT1.

Related Pathway
KEGG ORTHOLOGY:   
K01230
KEGG Pathway:   
ko00510  ko00513  ko01100  ko04141 
Reactome:   
R-HSA-6811438
Related Database
Organism:   
Homo sapiens
Uniprot:   
O60476 
NCBI GeneID:   
10905
GlyMap GeneID:   
10905
DNA aRefSeq:   
NM_006699.3 
Protein RefSeq:   
NP_006690.1 
BRENDA:   
Kinetic info.
OMIM number:   
604345
GeneCards database:   
MAN1A2
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Man(a1-2)Man(a1-?)
Product:  
     Man(a1-?)
Constraints:  
     nMan(a1-?)>=5&nMan(a1-?)<=7&!Man(a1-2)Man(a1-3)...Man(a1-6)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.00e-04    (pmol/cell • hr)       KM:    1.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4278 125-132 -0.473 97.0 -0.473 98.0 noncon
hsa-miR-4716-3p 124-131 -0.416 97.0 -0.416 98.0 noncon
hsa-miR-6794-5p 124-131 -0.411 97.0 -0.411 98.0 noncon
hsa-miR-6779-3p 3051-3058 -0.399 99.0 -0.358 99.0 noncon
hsa-miR-4632-3p 71-77 -0.375 90.0 -0.375 92.0 noncon
hsa-miR-656-5p 594-601 -0.372 95.0 -0.372 97.0 noncon
hsa-miR-203b-5p 46-53 -0.358 97.0 -0.358 98.0 noncon
hsa-miR-6881-3p 3435-3442 -0.355 97.0 -0.31 97.0 noncon
hsa-miR-3651 3053-3060 -0.322 94.0 -0.29 95.0 noncon
hsa-miR-4733-5p 1257-1264 -0.321 98.0 -0.305 99.0 noncon
hsa-miR-5697 1868-1875 -0.312 98.0 -0.294 99.0 noncon
hsa-miR-7160-3p 3737-3744 -0.311 96.0 -0.158 86.0 noncon
hsa-miR-4652-5p 2869-2876 -0.31 94.0 -0.283 94.0 noncon
hsa-miR-432-5p 1161-1168 -0.309 97.0 -0.293 97.0 noncon
hsa-miR-218-1-3p 373-380 -0.308 95.0 -0.308 96.0 noncon
hsa-miR-8081 525-532 -0.301 96.0 -0.301 97.0 noncon
hsa-miR-924 523-530 -0.299 96.0 -0.299 97.0 noncon
hsa-miR-4711-5p 3552-3559 -0.296 95.0 -0.259 95.0 noncon
hsa-miR-6718-5p 46-53 -0.294 96.0 -0.294 97.0 noncon
hsa-miR-4529-5p 363-369 -0.294 92.0 -0.294 95.0 noncon
hsa-miR-3913-3p 3031-3038 -0.292 94.0 -0.263 95.0 noncon
hsa-miR-378g 2587-2594 -0.29 97.0 -0.265 97.0 noncon
hsa-miR-4686 4570-4577 -0.287 95.0 -0.147 89.0 noncon
hsa-miR-4774-5p 5585-5592 -0.28 95.0 -0.143 87.0 noncon
hsa-miR-27b-5p 5607-5614 -0.276 95.0 -0.141 87.0 noncon
hsa-miR-3650 5236-5243 -0.274 94.0 -0.14 88.0 noncon
hsa-miR-501-3p 2680-2687 -0.273 95.0 -0.25 96.0 noncon
hsa-miR-7160-3p 2831-2838 -0.272 94.0 -0.249 94.0 noncon
hsa-miR-3173-5p 1797-1803 -0.267 94.0 -0.252 95.0 noncon
hsa-miR-6775-3p 1798-1805 -0.266 90.0 -0.251 92.0 noncon
hsa-miR-502-3p 2680-2687 -0.265 95.0 -0.243 96.0 noncon
hsa-miR-26a-2-3p 495-501 -0.258 95.0 -0.258 97.0 noncon
hsa-miR-3907 342-348 -0.257 91.0 -0.257 93.0 noncon
hsa-miR-6752-3p 2215-2222 -0.251 91.0 -0.237 93.0 noncon
hsa-miR-7154-3p 5836-5843 -0.25 95.0 -0.123 88.0 noncon