EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MAN2A2



General Information
Gene Symbol:   
MAN2A2
Protein name:   
Alpha-mannosidase 2x
Enzyme Class:   
Glycosidase
CAZy Family:   
GH38
E.C. No:   
3.2.1.114
Organs:   
Reaction (SNFG):   
MAN2A2 MAN2A2
H2O +
→
+
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Golgi resident mannosidase that cleaves the final two α1,3-Man and α1,6-Man during N-glycan processing.
• Man2α2 and Man2a1 act as isozymes, with similar α1,3/6-Man specificity.
• Absence of both is necessary for depeletion of complex N-glycans in mice, suggesting functional redundancy.
• Mice lacking MAN2A1 exhibit many of the features of humans lacking this enzyme activity including significant dyserythropoiesis, anemia, splenomegaly, and immature erythrocytes or reticulocytes in the peripheral blood, but no neurologic or behavioral symptoms, no reproductive problems, and no major histological findings in most tissues.
• Man2α2-deficient male mice are largely inferile, but females are normal.
• Swainsonine is a α-Mannosidase inhibitor.

Related Pathway
KEGG ORTHOLOGY:   
K01231
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-6811438
Related Database
Organism:   
Homo sapiens
Uniprot:   
P49641 
NCBI GeneID:   
4122
GlyMap GeneID:   
4122
DNA aRefSeq:   
NM_006122.3 
Protein RefSeq:   
NP_006113.2 
BRENDA:   
Kinetic info.
OMIM number:   
600988
GeneCards database:   
MAN2A2
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Man(a1-6)...Man(a1-6) or Man(a1-3)...Man(a1-6)
Product:  
     ...Man(a1-6) or ...Man(a1-6)
Constraints:  
     !Gal(b1-?)&nGlcNAc(b1-2)Man(a1-3)>=1&nGlcNAc(b1-?)=3
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.00e-04    (pmol/cell • hr)       KM:    2.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6878-3p 1892-1899 -0.437 98.0 -0.035 67.0 noncon
hsa-miR-1909-3p 1321-1328 -0.436 98.0 -0.436 98.0 noncon
hsa-miR-3618 1375-1382 -0.415 97.0 -0.415 98.0 noncon
hsa-miR-6722-3p 1321-1328 -0.399 98.0 -0.399 98.0 noncon
hsa-miR-6828-3p 2764-2771 -0.395 98.0 -0.032 68.0 noncon
hsa-miR-2682-5p 1388-1395 -0.391 99.0 -0.391 99.0 noncon
hsa-miR-449c-5p 1388-1395 -0.386 99.0 -0.386 99.0 noncon
hsa-miR-6891-3p 1337-1344 -0.383 97.0 -0.383 98.0 noncon
hsa-miR-6072 1337-1344 -0.378 98.0 -0.378 98.0 noncon
hsa-miR-34b-5p 1388-1395 -0.37 99.0 -0.37 99.0 noncon
hsa-miR-6887-3p 2735-2742 -0.367 95.0 -0.03 54.0 noncon
hsa-miR-4286 1782-1789 -0.357 98.0 -0.029 61.0 noncon
hsa-miR-1911-3p 2620-2627 -0.353 97.0 -0.029 59.0 noncon
hsa-miR-1184 630-637 -0.33 98.0 -0.33 98.0 noncon
hsa-let-7d-5p 2154-2161 -0.325 90.0 -0.027 42.0 noncon
hsa-miR-4458 2154-2161 -0.325 90.0 -0.027 42.0 noncon
hsa-miR-4500 2154-2161 -0.323 91.0 -0.027 45.0 noncon
hsa-let-7f-5p 2154-2161 -0.315 90.0 -0.026 44.0 noncon
hsa-miR-6826-3p 2736-2743 -0.309 94.0 -0.026 59.0 noncon
hsa-miR-4460 2137-2144 -0.308 93.0 -0.026 45.0 noncon
hsa-miR-371a-3p 1309-1316 -0.306 97.0 -0.306 98.0 noncon
hsa-miR-617 2789-2795 -0.304 95.0 -0.009 40.0 noncon
hsa-miR-6883-3p 1077-1084 -0.3 96.0 -0.3 97.0 noncon
hsa-miR-767-3p 2763-2770 -0.298 97.0 -0.025 53.0 noncon
hsa-miR-6778-3p 360-366 -0.288 96.0 -0.288 97.0 noncon
hsa-miR-6810-5p 816-823 -0.287 96.0 -0.287 97.0 noncon
hsa-miR-631 2515-2522 -0.283 93.0 -0.024 45.0 noncon
hsa-miR-4326 1340-1347 -0.282 95.0 -0.282 96.0 noncon
hsa-miR-501-5p 2773-2780 -0.272 96.0 -0.023 60.0 noncon
hsa-miR-548au-3p 1387-1393 -0.267 94.0 -0.267 95.0 noncon
hsa-miR-6752-3p 841-848 -0.266 92.0 -0.266 94.0 noncon
hsa-miR-18a-3p 195-202 -0.262 96.0 -0.262 97.0 noncon
hsa-miR-6865-5p 2479-2486 -0.252 93.0 -0.021 58.0 noncon
hsa-miR-6815-5p 2479-2486 -0.252 93.0 -0.021 58.0 noncon