FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST4



General Information
Gene Symbol:   
CHST4
Protein name:   
Carbohydrate sulfotransferase 4
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Five GlcNAc6ST enzymes have been found in humans.
• GlcNAc6ST-1 (CHST2) catalyzes the formation of 6-sulfo sialyl Lewis-X (6-sulfo sLeX, the major L-selectin ligand on HEV. This is also a major enzyme facilitating C6-GlcNAc sulfation of KS. CHST2 acts at the non-reducing end of GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3).
• GlcNAc6ST-2 (CHST4) is expressed on High Endothelial Cells of the lymph node (HEV). Both CHST2 and CHST4 can make 6-sulfo sialyl Lewis-X. It is also involved in the biosynthesis of MECA-79 epitope. CHST4 acts internally on LacNAc substrate.
• GlcNAc6ST-3 (CHST5) is a GlcNAc6ST that is functional in the intestines. It is highly expressed in colon and small intestine. CHST5 does not act to form KS, but it may attach sulfate to C6 on O-glycan core-2 glycan at the non-reducing end. As it is located in the intestines, it does not participate in the formation of the sulfated sLeX epitope.
• GlcNAc6ST-4/GST-5 (CHST7) is widely expressed in many organs. Less is known about this enzyme. It may contribute to C6 sulfation of GalNAc during chondroitin sulfate biosynthesis.
• GlcNAc6ST-5 (CHST6) is expressed in the cornea, and involved in the sulfation of keratin sulfate GAGs of the eye. The absence of this enzyme results in macular corneal dystrophy.

Related Pathway
KEGG ORTHOLOGY:   
K04746
KEGG Pathway:   
ko00533 
Reactome:   
R-HSA-913709
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NCG5 
NCBI GeneID:   
10164
GlyMap GeneID:   
10164
DNA aRefSeq:   
NM_001166395.1 
Protein RefSeq:   
NP_001159867.1 
BRENDA:   
Kinetic info.
OMIM number:   
-
GeneCards database:   
CHST4
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-3)[GlcNAc(b1-6)]GalNAc(a1-?)Ser|Thr or Gal(b1-3)[Gal(b1-4)GlcNAc(b1-6)]GalNAc(a1-?)Ser|Thr
Product:  
     Gal(b1-3)[GlcNAc(b1-6 [6S])]GalNAc(a1-?)Ser|Thr or Gal(b1-3)[Gal(b1-4)GlcNAc(b1-6 [6S])]GalNAc(a1-?)Ser|Thr
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6806-5p 70-77 -0.612 99.0 -0.612 99.0 noncon
hsa-miR-4661-3p 739-746 -0.545 99.0 -0.164 88.0 noncon
hsa-miR-4792 389-396 -0.491 99.0 -0.491 99.0 noncon
hsa-miR-450a-1-3p 664-671 -0.487 99.0 -0.487 99.0 noncon
hsa-miR-4766-3p 825-832 -0.463 99.0 -0.142 91.0 noncon
hsa-miR-138-5p 392-399 -0.444 97.0 -0.444 98.0 noncon
hsa-miR-10a-5p 226-233 -0.432 99.0 -0.432 99.0 noncon
hsa-miR-10b-5p 226-233 -0.432 99.0 -0.432 99.0 noncon
hsa-miR-4305 172-179 -0.401 97.0 -0.401 98.0 noncon
hsa-miR-2467-3p 599-606 -0.396 98.0 -0.396 99.0 noncon
hsa-miR-6509-3p 320-327 -0.394 98.0 -0.394 98.0 noncon
hsa-miR-181a-2-3p 319-326 -0.391 99.0 -0.391 99.0 noncon
hsa-miR-3138 346-353 -0.386 96.0 -0.386 97.0 noncon
hsa-miR-7114-5p 403-410 -0.385 99.0 -0.385 99.0 noncon
hsa-miR-1262 806-812 -0.381 98.0 -0.119 85.0 noncon
hsa-miR-3156-3p 458-465 -0.377 98.0 -0.377 98.0 noncon
hsa-miR-2355-5p 187-194 -0.37 98.0 -0.37 98.0 noncon
hsa-miR-26a-1-3p 752-759 -0.362 98.0 -0.113 87.0 noncon
hsa-miR-26a-2-3p 752-759 -0.362 98.0 -0.113 87.0 noncon
hsa-miR-3667-3p 450-457 -0.361 98.0 -0.361 98.0 noncon
hsa-miR-4475 690-696 -0.358 98.0 -0.358 99.0 noncon
hsa-miR-24-3p 62-68 -0.356 98.0 -0.356 98.0 noncon
hsa-miR-6884-5p 34-41 -0.356 98.0 -0.356 98.0 noncon
hsa-miR-6736-5p 806-812 -0.355 98.0 -0.111 85.0 noncon
hsa-miR-653-3p 218-225 -0.35 98.0 -0.35 99.0 noncon
hsa-miR-513a-5p 633-640 -0.344 98.0 -0.344 99.0 noncon
hsa-miR-5088-5p 423-429 -0.333 97.0 -0.333 98.0 noncon
hsa-miR-24-3p 81-87 -0.32 97.0 -0.32 98.0 noncon
hsa-miR-4302 110-117 -0.32 98.0 -0.32 98.0 noncon
hsa-miR-892a 311-318 -0.318 97.0 -0.318 98.0 noncon
hsa-miR-4701-3p 806-812 -0.315 97.0 -0.1 83.0 noncon
hsa-miR-3138 128-135 -0.313 92.0 -0.313 94.0 noncon
hsa-miR-3653-3p 703-710 -0.312 99.0 -0.312 99.0 noncon
hsa-miR-485-5p 34-41 -0.308 97.0 -0.308 98.0 noncon
hsa-miR-2113 538-545 -0.3 98.0 -0.3 98.0 noncon
hsa-miR-4762-3p 513-520 -0.298 98.0 -0.298 98.0 noncon
hsa-miR-4506 808-814 -0.296 96.0 -0.094 83.0 noncon
hsa-miR-7845-5p 689-695 -0.294 95.0 -0.294 96.0 noncon
hsa-miR-6809-5p 67-73 -0.292 94.0 -0.292 96.0 noncon
hsa-miR-6857-3p 38-45 -0.289 96.0 -0.289 97.0 noncon
hsa-miR-4668-5p 607-614 -0.282 97.0 -0.282 98.0 noncon
hsa-miR-4471 433-440 -0.281 93.0 -0.281 95.0 noncon
hsa-miR-4437 422-428 -0.281 92.0 -0.281 95.0 noncon
hsa-miR-6780b-5p 594-600 -0.28 93.0 -0.28 94.0 noncon
hsa-miR-4725-3p 594-600 -0.28 93.0 -0.28 94.0 noncon
hsa-miR-3678-3p 534-540 -0.279 96.0 -0.279 97.0 noncon
hsa-miR-1178-3p 201-207 -0.275 96.0 -0.275 97.0 noncon
hsa-miR-5579-3p 745-752 -0.268 96.0 -0.086 79.0 noncon
hsa-miR-24-3p 421-427 -0.266 95.0 -0.266 96.0 noncon
hsa-miR-7156-3p 576-582 -0.265 94.0 -0.265 96.0 noncon
hsa-miR-4756-3p 77-84 -0.263 98.0 -0.263 99.0 noncon
hsa-miR-1295b-3p 296-302 -0.261 91.0 -0.261 94.0 noncon
hsa-miR-885-3p 641-647 -0.256 95.0 -0.256 96.0 noncon
hsa-miR-4708-3p 66-72 -0.254 92.0 -0.254 94.0 noncon
hsa-miR-3184-3p 278-284 -0.25 96.0 -0.25 97.0 noncon