GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
TSTA3



General Information
Gene Symbol:   
TSTA3
Protein name:   
GDP-L-fucose synthase
Enzyme Class:   
CAZy Family:   
E.C. No:   
1.1.1.271
Tissues:   
all, Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   

GDP-4-keto-6-deoxy-D-mannose → GDP-fucose
Reaction (IUBMB):   
No specified reaction.
About

• Synthesizes GDP-fucose GDP-4-keto-6-deoxy-D-mannose via an epimerization and reduction reaction which utilizes NADPH.
• Tissue-specific transplantation antigen (TSTA3) is a known cancer biomarker at the transcriptional level

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-6787639
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q13630 
NCBI GeneID:   
7264
GlyMap GeneID:   
7264
DNA aRefSeq:   
NM_001317783.1 
Protein RefSeq:   
NP_001304712.1 
BRENDA:   
Kinetic info.
OMIM number:   
137020
GeneCards database:   
TSTA3
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     GDP-4-keto-6-deoxy-D-mannose
Product:  
     GDP-fucose
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 60 50 40 30 20 10 0 RPKM
Expression Data in Cells
No expression data available.
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
TSTA3/hsa-miR-380-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AGCGGCCACCACCC GUCAACC C...
3'   CGCGUACAAGAUAC CAGUUGG U
 7mer-m8 64-70 -0.277 93.0 -0.277 95.0 noncon