GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST8SIA4



General Information
Gene Symbol:   
ST8SIA4
Protein name:   
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.8
Organs:   
Reaction (SNFG):   
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• Polysialyltransferase ST8SIA4 adds sialic acids by α2,8-linkage on α2,3- or α2,6-linked sialic acid
• Polysialic acid on NCAM in the brain is synthesized by two polysialyltransferases, ST8SIA2 and ST8SIA4
• The majority of polysialic acid expressed in adult brain is lost in ST8SIA4-deficient mice, but polysialic acid is remained in premature neurons and migrating neurons expressing ST8SIA2. Lack of polysialic acid in double-knockout mice causes severe abnormalities in brain development such as neural cell death, aberrant neural cell migration, and differentiation.
• Polysialic acid is detected in many cancer cell lines. ST8SIA4 mRNA is detectable in human neuroblastoma, small and non-small cell lung carcinoma, and glioma. Here, polySia is thought to affect adhesive property of tumor cells and promote metastasis and migration of cancer cells in vivo. Thus, polysialic acid regulation may be an important target on tumor cells.
• Restricted to selected proteins (NCAM, SynCAM-1)

Related Pathway
KEGG ORTHOLOGY:   
K06614
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q92187 
NCBI GeneID:   
7903
GlyMap GeneID:   
7903
DNA aRefSeq:   
NM_005668.5 
Protein RefSeq:   
NP_005659.1 
BRENDA:   
Kinetic info.
OMIM number:   
602547
GeneCards database:   
ST8SIA4
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type)
Compartment:  
     
Substrate:  
     NeuAc(a2-?)
Product:  
     NeuAc(a2-8)NeuAc(a2-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
ST8SIA4/hsa-miR-4735-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...UCUCAUUUAAGUA GCACCUU U...
3'   UACAGAUUAAACU CGUGGAA A
 7mer-m8 4364-4370 -0.301 96.0 -0.301 97.0 noncon