GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
PAPSS2



General Information
Gene Symbol:   
PAPSS2
Protein name:   
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.7.7.4
Tissues:   
Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   

ATP → PAPS
Reaction (IUBMB):   
No specified reaction.
About

• PAPSS1/2 have two functions of phosphorylation of ADP and sulfation of ATP. These are crucial for the formation of PAPS, which is the source for sulfates in glycan modifications.
• PAPSS2 deficiency has been shown to cause physical deformities later in life.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-174362
Related Database
Organism:   
Homo sapiens
Uniprot:   
O95340 
NCBI GeneID:   
9060
GlyMap GeneID:   
9060
DNA aRefSeq:   
NM_001015880.1 
Protein RefSeq:   
NP_001015880.1 
BRENDA:   
Kinetic info.
OMIM number:   
603005
GeneCards database:   
PAPSS2
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     ATP
Product:  
     PAPS
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
PAPSS2/hsa-let-7a-2-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...GAUUGUGUCCUCUU CUGUACAA ...
3'   CCUUUCGAUCCUCC GACAUGU C
 8mer 423-430 -0.305 98.0 -0.208 96.0 noncon
5'...UAUUUUACCUCAUG CUGUACAA ...
3'   CCUUUCGAUCCUCC GACAUGU C
 8mer 513-520 -0.191 93.0 -0.132 90.0 noncon
5'...AAUGAUUAUUUCUA UGUACAA C...
3'   CCUUUCGAUCCUCCG ACAUGU C
 7mer-A1 1689-1695 -0.147 88.0 -0.102 86.0 noncon