GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MGAT4B



General Information
Gene Symbol:   
MGAT4B
Protein name:   
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT54
E.C. No:   
2.4.1.145
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• MGAT4 catalyzes β1-4GlcNAc linkage to the GlcNAcβ1-2Manα1,3 arm of N-glycans.
• Two isoenzymes MGAT4A and MGAT4B, both of which have different tissue distributions, contribute to cellular MGAT4/GnT-IV activity. A third enzyme has also been reported but is not characterized.
• MGAT4 and MGAT5 both contribute to N-glycan branching, and they are ubiquitously expressed in various organs.
• N-glycan branching due to GnT-IVa may be aberrantly upregulated in some type of tumors and this can serve as a biomarker.

Related Pathway
KEGG ORTHOLOGY:   
K00738
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-975577
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9UQ53 
NCBI GeneID:   
11282
GlyMap GeneID:   
11282
DNA aRefSeq:   
NM_014275.4 
Protein RefSeq:   
NP_055090.1 
BRENDA:   
Kinetic info.
OMIM number:   
604561
GeneCards database:   
MGAT4B
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-2)Man(a1-3)
Product:  
     GlcNAc(b1-2)[GlcNAc(b1-4)]Man(a1-3)
Constraints:  
     !GlcNAc(b1-4)...Man(b1-4)&!Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    1.00e-05    (pmol/cell • hr)       KM:    3.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
MGAT4B/hsa-miR-6715b-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...CGGCCCCCACACU GUGCCUGA ...
3'   ACGGUUUGGUCAG CACGGAC A
 8mer 221-228 -0.323 98.0 -0.323 98.0 noncon