GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HK3



General Information
Gene Symbol:   
HK3
Protein name:   
Hexokinase-3
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.7.1.1
Organs:   
Reaction (SNFG):   

Glc or Fruc or Man → Glc(6P) or Fruc(6P) or Man(6P)
Reaction (IUBMB):   
No specified reaction.
About

• Hexokinase 3 phosphorylates glucose and fructose.
• This enzyme catalyzes the first step of glycosylsis, converting glucose to glucose-1-phosphate.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-6798695
Related Database
Organism:   
Homo sapiens
Uniprot:   
P52790 
NCBI GeneID:   
3101
GlyMap GeneID:   
3101
DNA aRefSeq:   
NM_002115.2 
Protein RefSeq:   
NP_002106.2 
BRENDA:   
Kinetic info.
OMIM number:   
142570
GeneCards database:   
HK3
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     Glc or Fruc or Man
Product:  
     Glc(6P) or Fruc(6P) or Man(6P)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 60 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 10 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000292432 ENST00000506834 ZNF467 ESRRA ZNF263 GATA2 USF1 ASCL1 MYOD1 MYF5 ERG TAL1 NR2F6 ZNF618 MYC PPARG HINFP CLOCK ZBTB42 CRY1 KMT2A RELA JUN E2F6 GATA6 RUNX1 GRHL3 ZNF263 KMT2A ZNF316 MAFG MAFK NFE2 FLI1 ZNF263 RUNX1 KMT2A ERG GATA2 GATA2 LYL1 TCF12 TCF12 SKI MECOM NFE2 NCOR1 STAT5A TCF3
miRNA-GlycoEnzyme Interactome {ref. 9}
HK3/hsa-miR-5787
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...GUCUGCCUGUUU CCCAGCCA ...
3'   UGGAGGGGCGCG GGGUCGG G
 8mer 68-75 -0.366 98.0 -0.366 99.0 noncon
5'...UUCCCAGCCAGG CCCAGCCA ...
3'   UGGAGGGGCGCG GGGUCGG G
 8mer 78-85 -0.279 97.0 -0.279 97.0 noncon