GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GBA



General Information
Gene Symbol:   
GBA
Protein name:   
Lysosomal acid glucosylceramidase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GH30
E.C. No:   
3.2.1.45
Organs:   
Reaction (SNFG):   

Glc(b1-?)Cer → Cer
Reaction (IUBMB):   
No specified reaction.
About

• Lysosomally localized glucosylceramidase, responsible for the breakdown of ceramide-linked glycans
• Mutations in GBA give rise to Parkinson?s disease, and can result in a lysosomal storage disorder called Gaucher disease.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
P04062 
NCBI GeneID:   
2629
GlyMap GeneID:   
2629
DNA aRefSeq:   
NM_000157.3 
Protein RefSeq:   
NP_000148.2 
BRENDA:   
Kinetic info.
OMIM number:   
606463
GeneCards database:   
GBA
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     Glc(b1-?)Cer
Product:  
     Cer
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 500 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
GBA/hsa-miR-6736-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AAGGGGUAAAGAU GGAGCUG G...
3'   GACACCCAUCUCU CCUCGAC U
 7mer-m8 1192-1198 -0.254 95.0 -0.203 94.0 noncon
5'...GCGUGGACGCUGG GGAGCUG C...
3'   GACACCCAUCUCU CCUCGAC U
 7mer-m8 2276-2282 -0.053 67.0 -0.043 73.0 noncon
5'...UCUGCCCCUUGCU GAGCUGA C...
3'   GACACCCAUCUCUC CUCGAC U
 7mer-A1 2212-2218 -0.01 33.0 -0.008 50.0 noncon