GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT8



General Information
Gene Symbol:   
FUT8
Protein name:   
Alpha-(1,6)-fucosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT23
E.C. No:   
2.4.1.68
Organs:   
Reaction (SNFG):   
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• Mediates the α1,6-fucosylation of N-glycans in many eukaryotes, but not plants and fungi.
• FUT8 primarily acts on complex and hybrid N-glycans as it requires GlcNAc-beta attached to Asn, and also the β1,2 GlcNAc residue linked to the α1,3 mannose arm.
• In some instances this may be cancer specific as core fucosylation is observed specifically on hepatocellular carcinoma (HCC), but not in chronic liver disease.
• Depletion of the core fucose in human IgG1 enhances the antibody-dependent cellular cytotoxicity (ADCC) activity.
• FUT8 activity is inhibited by bisecting GlcNAc (produced by MGAT3) and also terminal fucosylation.
• FUT8 is highly expressed in brain tissue, human blood platelet, cancer tissues and cancer cell lines.

Related Pathway
KEGG ORTHOLOGY:   
K00717
KEGG Pathway:   
ko00510  ko00513  ko00533  ko01100  ko05202 
Reactome:   
R-HSA-975578
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9BYC5 
NCBI GeneID:   
2530
GlyMap GeneID:   
2530
DNA aRefSeq:   
NM_004480.4 
Protein RefSeq:   
NP_004471.4 
BRENDA:   
Kinetic info.
OMIM number:   
602589
GeneCards database:   
FUT8
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     ...GlcNAc(b1-?)Asn
Product:  
     ...[Fuc(a1-6)]GlcNAc(b1-?)Asn
Constraints:  
     nGlcNAc(b1-2)Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(b1-?)>=1&!GlcNAc(b1-4)...Man(b1-4)&!Fuc(a1-3)&!Fuc(a1-3)...GlcNAc(b1-?)Asn
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.80e-05    (pmol/cell • hr)       KM:    2.50e+07    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
FUT8/hsa-miR-1226-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'... ACAAGGGCUGCAAU GCCCUCA U...
3'   GGGGUAGGUCCGGACGUA CGGGAGU G
 7mer-m8 197-203 -0.264 95.0 -0.264 96.0 noncon