GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT6



General Information
Gene Symbol:   
FUT6
Protein name:   
Alpha-(1,3)-fucosyltransferase 6
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT10
E.C. No:   
2.4.1.65
Organs:   
Reaction (SNFG):   
+
→
+ GDP
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• FUT6 utilizes solely type 2 LacNAc but not type 1 LacNAc, Sialylated oligosaccharides are good substrates as well as non-sialylated ones.
• Thus, FUT6 is able to synthesize LewisX and sialyl LewisX, but not sialyl Lewisa or Lewisa.
• The enzyme creates these epitopes on plasma proteins (plasma, milk, saliva) and also epithelial cells (liver, kidney, colon, small intestine, bladder, uterus, and salivary glands).
• It is not expressed in rodents.
• May be associated with the expression of LeX and sLeX antigen on plasma/liver-secreted proteins after inflammation mediated by IL-1β and IL-6.
• FUT6 is widely expressed in cancer cells, and may be partly responsible for the synthesis of sialyl LewisX in these cells (along with FUT3, FUT5).
• It may regulate cancer cell growth via the PI3K/Akt signaling pathway.

Related Pathway
KEGG ORTHOLOGY:   
K07634
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
P51993 
NCBI GeneID:   
2528
GlyMap GeneID:   
2528
DNA aRefSeq:   
NM_000150.2 
Protein RefSeq:   
NP_000141.1 
BRENDA:   
Kinetic info.
OMIM number:   
136836
GeneCards database:   
FUT6
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?) or NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-?)
Product:  
     Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?) or NeuAc(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?)
Constraints:  
     !GlcNAc(b1-6)[GlcNAc(b1-3)]Gal(b1-4)@GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.75e-06    (pmol/cell • hr)       KM:    1.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000286955 ENST00000527106 ENST00000592563 SP1 GLIS1 ZNF264 MAZ TP73 ASCL1 MITF ELF4 ZBTB40 RUNX1 PPARG GATA2 ZFP3 EBF1 SPIB ZBTB7A FOXA3 TFAP4 FOXA2 JUND RCOR1 TFAP2C RCOR2
miRNA-GlycoEnzyme Interactome {ref. 9}
FUT6/hsa-miR-4742-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'... ACCUAAGGACUCGU UUGCCUGA ...
3'   AGACAUUUAUAGGGA AACGGAC U
 8mer 87-94 -0.315 98.0 -0.315 99.0 noncon
5'... CCUGAAGCUUCACC UGCCUGA G...
3'   AGACAUUUAUAGGGAA ACGGAC U
 7mer-A1 104-110 -0.105 84.0 -0.105 89.0 noncon