GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 

Pathway Map

FUT3



General Information
Gene Symbol:   
FUT3
Protein name:   
Galactoside 3(4)-L-fucosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   
+
+ GDP
+
+ GDP
+
+ GDP
Reaction (IUBMB):   
No specified reaction.

About

• FUT3 exhibits broad specificity synthesizing all Lewis antigens, Lea, Leb, LeX, LeY, sialyl Lea (sLea), and sLeX. Lea and Leb are histo-blood group antigens which are present not only on erythrocytes but also in gastrointestinal, pancreatic, and other tissues in the body.
• FUT3 α1-4-fucosyltransferase activity creates Lewis-a and Lewis-b on Type I (Galβ3GlcNAc, its preferred substrate) by transferring fucose to GlcNAc on either Type I chain (to make Lewis-a) or on H-antigen formed on Type I chain (to make Lewis-b). It is the only enzyme making Lea, Leb, and sLea in vivo, since FUT5 is a silent enzyme that is not expressed in any tissue.
• FUT3 can also act on Type II (Galβ4GlcNAc) lactosamine chains to create LewisX, LewisY and sialyl Lewis-X type structures.
• FUT3 also forms the stage-specific embryonal antigen-1 (SSEA-1) in humans.
• FUT3, FUT4, FUT5, and FUT6 exhibit similar specificity for preferential fucose transfer to the inner GlcNAc on polylactosamine chains. FUT9 prefers the terminal modifications to create terminal LeX.
• The sequence of FUT3 is similar to that of FUT5 and FUT6.
• Mouse do not express FUT3 and thus are devoid of Lea Leb type structures. Mouse SSEA-1 is created by FUT9 instead of FUT3.
• FUT3 is widely expressed in epithelial cells of the digestive tract, including salivary gland, mammary gland, esophagus, stomach, pancreas, small intestine, colon, and rectum. FUT3 is not expressed in hematopoietic cells. Lea- and Leb-active glycolipid antigens on erythrocytes are not synthesized by erythrocytes themselves and are probably synthesized elsewhere in the digestive organs by FUT3; and then absorbed by RBCs.
• CA19-9, tumor biomarker, recognizes the sLea epitope on mucins. Individuals who are FUT3 negative will not express this biomarker.
• Similarly, the expression of DU-PAN-2 is also dependent on the activity of genes forming sLec.


Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
BRENDA:   
OMIM number:   
-
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     ...Gal(b1-4)GlcNAc(b1-?) or ...Gal(b1-3)GlcNAc(b1-?) or GalNAc(b1-3)GlcNAc(b1-?)
Product:  
     ...Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?) or ...Gal(b1-3)[Fuc(a1-4)]GlcNAc(b1-?) or GalNAc(b1-3)[Fuc(a1-4)]GlcNAc(b1-?)
Constraints:  
     !GlcNAc(b1-6)[GlcNAc(b1-3)]Gal(b1-4)@GlcNAc(b1-?)

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.75e-06    (pmol/cell • hr)       KM:    1.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data

miRNA-GlycoEnzyme Interactome {ref. 9}
FUT3/hsa-miR-323a-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AAGUUCAAGCCCAG GACCACCA ...
3'   CGCUUGCGCGGUGC CUGGUGG A
8mer 611-618 -0.383 97.0 -0.383 98.0 noncon