GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT11



General Information
Gene Symbol:   
FUT11
Protein name:   
Alpha-(1,3)-fucosyltransferase 11
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT10
E.C. No:   
2.4.1.152
Organs:   
Reaction (SNFG):   
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• FUT 10 and 11 are invertebrate and plant core α3-fucosyltransferases that are expressed in the ER (and not Golgi).
• They attach α(1,3)Fuc to the innermost core GlcNAc of the N-glycans on conalbumin glycopeptide substrates and also synthetic biantennary N-glycans.
• It is generally accepted that the core α3-fucosylation mediated by these enzymes does not occur in humans.
• However the wide spread expression of core α(1,3)Fuc in pollen, foods, parasites and insect venom may result in IgE dependent allergic reactions in humans.

Related Pathway
KEGG ORTHOLOGY:   
K11257
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q495W5 
NCBI GeneID:   
170384
GlyMap GeneID:   
170384
DNA aRefSeq:   
NM_001284194.1 
Protein RefSeq:   
NP_001271123.1 
BRENDA:   
Kinetic info.
OMIM number:   
-
GeneCards database:   
FUT11
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     ...GlcNAc(b1-?)Asn
Product:  
     ...[Fuc(a1-3)]GlcNAc(b1-?)Asn
Constraints:  
     nGlcNAc(b1-2)Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(b1-?)Asn>=1&!GlcNAc(b1-4)^Man(b1-4)&!Fuc(a1-6)...GlcNAc(b1-?)Asn
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.80e-05    (pmol/cell • hr)       KM:    2.50e+07    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
FUT11/hsa-miR-7703
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'... AGAUUAUUGGGAAA UGUACUG A...
3'   GGACCCUCUUCCGGUC UCACGU U
 6mer 4161-4167 -0.253 96.0 -0.204 96.0 noncon
5'... UGGCUGCAUCCCCA AAUCUGA C...
3'   GGACCCUCUUCCGGUC UCACGU U
 7mer-A1 1823-1829 -0.125 87.0 -0.112 90.0 noncon
5'... GUGGAUCAUGGCAG UGAGAGCU ...
3'   GGACCCUCUUCCGGU CUCACGU U
 8mer 4649-4656 -0.126 87.0 -0.102 89.0 noncon
5'... AAAAAGGUGGGGGC ACCUGGG C...
3'   GGACCCUCUUCCGGUC UCACGU U
 6mer 2394-2400 -0.109 84.0 -0.097 88.0 noncon
5'... CUAAUGGUCUCCCU GCUCAUU A...
3'   GGACCCUCUUCCGGUC UCACGU U
 6mer 1767-1773 -0.071 76.0 -0.064 83.0 noncon
5'... CCCACUUCAGCCUC CCAAGUA G...
3'   GGACCCUCUUCCGGUC UCACGU U
 7mer-A1 1214-1220 -0.02 41.0 -0.018 64.0 noncon
5'... AAGUAGUCUCAGGC UGGGCAC G...
3'   GGACCCUCUUCCGGUC UCACGU U
 6mer 2089-2095 -0.02 41.0 -0.018 64.0 noncon
5'... GGAUUACAGGUGUG AGCCACC G...
3'   GGACCCUCUUCCGGUC UCACGU U
 6mer 1986-1992 -0.01 30.0 -0.009 53.0 noncon