GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST9



General Information
Gene Symbol:   
CHST9
Protein name:   
Carbohydrate sulfotransferase 9
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Catalyzes C4 sulfation of GalNAc in GalNAc(β1-4)GlcNAcβ on N- and O-glycans.
• Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin

Related Pathway
KEGG ORTHOLOGY:   
K09673
KEGG Pathway:   
ko00513  ko01100 
Reactome:   
R-HSA-2022870
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q7L1S5-1 
NCBI GeneID:   
83539
GlyMap GeneID:   
83539
DNA aRefSeq:   
NM_001256316.1 
Protein RefSeq:   
NP_001243245.1 
BRENDA:   
Kinetic info.
OMIM number:   
610191
GeneCards database:   
CHST9
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type)
Compartment:  
     
Substrate:  
     GalNAc(b1-4)GlcNAc(b1-?)
Product:  
     GalNAc(b1-4 [4S])GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000581714 ENST00000618847 CTCF KDM5C NR2F2 JUND RXRA ZBTB26 GATAD2A RARA RXRB SMAD4 SOX13 SOX13 CREB1 YY1 ATF3 HMG20A TEAD4 TEAD4 RUNX1 MAX DMAP1 USF1 HNF1B HMG20A CEBPG CEBPG MIXL1 SOX5 GATA4 HNF1A TEAD1 FOXA2 BCL6 HNF4A SOX13 ZNF7 ZNF3 ZKSCAN1
miRNA-GlycoEnzyme Interactome {ref. 9}
CHST9/hsa-miR-1257
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AACAAAGACCAGU CAUUCACA ...
3'   CCAGUCUUGGGUA GUAAGUG A
 8mer 74-81 -0.446 99.0 -0.446 99.0 noncon
5'...CAGUAGGAAUGGA CAUUCAC U...
3'   CCAGUCUUGGGUA GUAAGUG A
 7mer-m8 778-784 -0.197 91.0 -0.197 94.0 noncon
5'...AUUGGCCAGUGGA CAUUCAC C...
3'   CCAGUCUUGGGUA GUAAGUG A
 7mer-m8 199-205 -0.174 87.0 -0.174 92.0 noncon