GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST1



General Information
Gene Symbol:   
CHST1
Protein name:   
Carbohydrate sulfotransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
2.8.2.21
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Keratan sulfate (KS) is composed of repeating disaccharide LacNAc units (Galβ1-4GlcNAc) with C6 sulfation of both Gal and GlcNAc. GlcNAc sulfation often exceeds Gal sulfation suggesting that GlcNAc sulfation may occur first.
• CHST1 has C6 Gal sulfation properties (both for unsulfated LacNAc substrate and also sulfated KS).
• In addition to KS, this enzyme may also contribute to the formation of glycoproteins bearing C6-Gal sulfated sialyl N-acetyllactosamine or sulfated Sialyl-Lewis X oligosaccharides. These glycan structures can act as selectin ligands during lymphocyte homing to HEV (high endothelial venules).
• The enzyme does not act on CS, DS or HS.
• In vitro synthesis of KS has been achieved by mixing some of these sulfotransferases with B4GalT1,4, along with B3GNT2 and 7 (for lactosamine chain extension).

Related Pathway
KEGG ORTHOLOGY:   
K01022
KEGG Pathway:   
ko00533 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
O43916 
NCBI GeneID:   
8534
GlyMap GeneID:   
8534
DNA aRefSeq:   
NM_003654.5 
Protein RefSeq:   
NP_003645.1 
BRENDA:   
Kinetic info.
OMIM number:   
603797
GeneCards database:   
CHST1
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-3 [6S]) or NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-? [6S]) or NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-4 [6S])GlcNAc(b1-3 [6S]) or NeuAc(a2-3)Gal(b1-4 [6S])GlcNAc(b1-? [6S]) or NeuAc(a2-3)Gal(b1-4 [6S])GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
CHST1/hsa-miR-506-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...CGUUUUUAACUGU UGCCUUA U...
3'   AGAUGAGUCUUCCC ACGGAA U
 7mer-A1 71-77 -0.308 95.0 -0.308 96.0 con
5'...CAGCUCUAGCAAA UGCCUUAA ...
3'   AGAUGAGUCUUCC CACGGAA U
 8mer 1853-1860 -0.262 91.0 -0.001 18.0 noncon