GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GNT5



General Information
Gene Symbol:   
B3GNT5
Protein name:   
Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.206
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• B3GnT5 is a lactotriaosylceramide (Lc3Cer) synthase and is considered to be important for synthesizing lacto-/neolactoseries glycolipids.
• B3GnT5 prefers shorter LacNAc substrates (2LN and 3LN), but no longer ones.
• Disruption of the B3gnt5 gene in mice leads to pre-implantation lethality. Defects in B-cell function and lipid raft formation are also reported.

Related Pathway
KEGG ORTHOLOGY:   
K03766
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-913709
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9BYG0 
NCBI GeneID:   
84002
GlyMap GeneID:   
84002
DNA aRefSeq:   
NM_032047.4 
Protein RefSeq:   
NP_114436.1 
BRENDA:   
Kinetic info.
OMIM number:   
615333
GeneCards database:   
B3GNT5
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)Glc(b1-?)Cer or Gal(b1-4)
Product:  
     GlcNAc(b1-3)Gal(b1-4)Glc(b1-?)Cer or GlcNAc(b1-3)Gal(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.70e-06    (pmol/cell • hr)       KM:    7.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000326505 ENST00000462559 ENST00000465010 CREB3 ZNF584 SMARCC1 ZNF589 ZNF175 NR2C2 MITF MAX ZNF143 USF2 NFE2 TFAP2C JUN E2F6 MYC CREM TAL1 E2F4 E2F4
miRNA-GlycoEnzyme Interactome {ref. 9}
B3GNT5/hsa-miR-4764-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AAUCACGUCUUAC CACAUCCA ...
3'   UCUAUUGAGGAAG GUGUAGG U
 8mer 568-575 -0.271 92.0 -0.271 94.0 noncon