GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GNT2



General Information
Gene Symbol:   
B3GNT2
Protein name:   
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.149
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• This is the principal enzyme forming polylactosamine [Gal(β1-4)GlcNAc(β1-3)]n structures or "i-antigen" on N- and O-glycans (e.g. core-2 glycans), and glycolipids. It also contributes to chain extension in keratan sulfates.
• Long polylactosamine chains on N-glycans was greatly reduced in the tissues of B3gnt2-/- mice.
• It is structurally similar to β1,3-galactosyltransferases.
• Polylactosamine structures are recognized by LEL/tomato lectin and PWM (pokeweed lectin).
• B3GnT2 and B3GnT8 may form a heterocomplex to facilitate polyLacNAc extension.
• Broad, ubiquitous tissue distribution.
• Lack of the enzyme activity in human results in autoimmune diseases.

Related Pathway
KEGG ORTHOLOGY:   
K00741
KEGG Pathway:   
ko00533  ko00601  ko01100 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NY97 
NCBI GeneID:   
10678
GlyMap GeneID:   
10678
DNA aRefSeq:   
NM_001319075.1 
Protein RefSeq:   
NP_001306004.1 
BRENDA:   
Kinetic info.
OMIM number:   
605581
GeneCards database:   
B3GNT2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL, GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.70e-06    (pmol/cell • hr)       KM:    7.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000301998 ENST00000405767 RUNX1 KLF9 USF1 USF2 ZNF341 SMAD2 NFE2 ZXDC ZXDC RCOR1 PITX3 ZBTB6 ZBTB6 GLIS1 E2F6 E2F4 KLF9 TFAP4 TEAD4 NFE2 KLF1 NRF1 ZNF18 MYC NFYB AR USF2 KMT2B KLF4 CREM GATA6 ATF1 USF1 SRF STAT5A MITF
miRNA-GlycoEnzyme Interactome {ref. 9}
B3GNT2/hsa-miR-3616-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'... UAAGUGAUUUUGUU UGCCCUC U...
3'   CGGACGUAGUACUUU ACGGGAG C
 7mer-m8 314-320 -0.323 94.0 -0.323 96.0 noncon