GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG9



General Information
Gene Symbol:   
ALG9
Protein name:   
Alpha-1,2-mannosyltransferase ALG9
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT22
E.C. No:   
2.4.1.259
Organs:   
Reaction (SNFG):   
+
→
+ P-Dol
+
→
+ P-Dol
Reaction (IUBMB):   
No specified reaction.
About

• Extension of Man5GlcNAc2-P-P-Dol in the ER lumen involves the addition of four Man (by ALG3 [α(1,3)ManT], ALG9 [α(1,2)ManT], ALG12 [α(1,6)ManT] followed by ALG9 [α(1,2)ManT] again) and three Glc residues (by ALG6 [α(1,3)GlcT], ALG8 [α(1,3)GlcT] and ALG10 [α(1,2)GlcT]).
• In all instances the transfer uses either Dol-P-Man or Dol-P-Glc as donor.
• Dol-P-Man is formed from GDP-Man using enzymes DPM1,2,3
• Dol-P-Glc is formed from UDP-Glc using ALG5

Related Pathway
KEGG ORTHOLOGY:   
K03846
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9H6U8 
NCBI GeneID:   
79796
GlyMap GeneID:   
79796
DNA aRefSeq:   
NM_001077690.1 
Protein RefSeq:   
NP_001071158.1 
BRENDA:   
Kinetic info.
OMIM number:   
606941
GeneCards database:   
ALG9
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Man(a1-3)Man(a1-6) or Man(a1-6)...Man(a1-6)
Product:  
     Man(a1-2)Man(a1-3)Man(a1-6) or Man(a1-2)Man(a1-6)...Man(a1-6)
Constraints:  
     nMan>6&nMan(a1-6)=2
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
ALG9/hsa-miR-7973
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...GUAGUCAAUCUG GGGUCACA ...
3'   CAUUAAUAAGAU CCCAGUG U
 8mer 1570-1577 -0.292 93.0 -0.011 43.0 noncon
5'...UUAGUUGCCUAA GGUCACA C...
3'   CAUUAAUAAGAUC CCAGUG U
 7mer-A1 1225-1231 -0.053 54.0 -0.002 30.0 noncon