GlycoEnzDB

General Information

Gene Symbol:

ALG12

Protein name:

Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase

Enzyme Class:

Glycosyltransferase

CAZy Family:

GT22

E.C. No:

2.4.1.260

Organs:

Reaction (SNFG):

+

→

+

P-Dol

Reaction (IUBMB):

No specified reaction.

About

• Extension of Man5GlcNAc2-P-P-Dol in the ER lumen involves the addition of four Man (by ALG3 [α(1,3)ManT], ALG9 [α(1,2)ManT], ALG12 [α(1,6)ManT] followed by ALG9 [α(1,2)ManT] again) and three Glc residues (by ALG6 [α(1,3)GlcT], ALG8 [α(1,3)GlcT] and ALG10 [α(1,2)GlcT]).
• In all instances the transfer uses either Dol-P-Man or Dol-P-Glc as donor.
• Dol-P-Man is formed from GDP-Man using enzymes DPM1,2,3
• Dol-P-Glc is formed from UDP-Glc using ALG5

Related Pathway

KEGG ORTHOLOGY:

K03847

KEGG Pathway:

ko00510 ko00513 ko01100

Reactome:

R-HSA-446193

Related Database

Organism:

Homo sapiens

Uniprot:

Q9BV10

NCBI GeneID:

79087

GlyMap GeneID:

79087

DNA aRefSeq:

NM_024105.3

Protein RefSeq:

NP_077010.1

BRENDA:

Kinetic info.

OMIM number:

607144

GeneCards database:

ALG12

Enzyme Specificity

Glycan Type:

N-linked

Compartment:

ER

Substrate:

Man(a1-2)Man(a1-3)Man(a1-6)

Product:

Man(a1-2)Man(a1-3)[Man(a1-6)]Man(a1-6)

Constraints:

nMan=7&nMan(a1-6)=1

Simulation Parameters

Mechaelis-Menten parameters (suggested)
VM: (pmol/cell • hr) KM: (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)

ER	Cis-	Medial-	Trans-	TGN

Expression Data in Tissue {ref. 5} Source from NCBI gene database

Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database

Transcription factor {ref. 10}

miRNA-GlycoEnzyme Interactome {ref. 9}

ALG12/hsa-miR-6867-5p

miRNA Target	Site Type	Pos.	Context Score	Frac.	Wt. Context	Frac.	Conserved
5'... ACCAUGCAUACAUG CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	840-847	-0.727	99.0	-0.018	37.0	noncon
5'... CCAUGCAUACACAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	937-944	-0.764	99.0	-0.019	37.0	noncon
5'... CAUUACCAUGCAUC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	972-979	-0.769	99.0	-0.019	37.0	noncon
5'... CCAUGCAUACACAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1080-1087	-0.76	99.0	-0.018	37.0	noncon
5'... CAUUACCAUGCAUC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1115-1122	-0.765	99.0	-0.019	37.0	noncon
5'... CCAUGCAUACACAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1252-1259	-0.756	99.0	-0.018	37.0	noncon
5'... ACAUUACCAUGCAU ACACACA G... 3' AGGGAAGAAGGAGAUG UGUGUG U	7mer-A1	1286-1292	-0.702	99.0	-0.017	36.0	noncon
5'... CCAUGCAUACACAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1491-1498	-0.75	99.0	-0.018	37.0	noncon
5'... CAUUACCAUGCAUC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1526-1533	-0.75	99.0	-0.018	37.0	noncon
5'... CCAUGCAUACACAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1658-1665	-0.745	99.0	-0.018	37.0	noncon
5'... CCAUGCAUACACAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1697-1704	-0.749	99.0	-0.018	37.0	noncon
5'... CCAUGCAUGCACAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1767-1774	-0.715	99.0	-0.018	36.0	noncon
5'... ACGUUACCAUGCAU ACACACA G... 3' AGGGAAGAAGGAGAUG UGUGUG U	7mer-A1	1801-1807	-0.682	99.0	-0.017	36.0	noncon
5'... CCAUGCAUACACAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1870-1877	-0.746	99.0	-0.018	37.0	noncon
5'... ACAUUACCGUGCAU ACACACA G... 3' AGGGAAGAAGGAGAUG UGUGUG U	7mer-A1	1904-1910	-0.662	99.0	-0.017	36.0	noncon
5'... ACAUUACCAUGCAU ACACACA G... 3' AGGGAAGAAGGAGAUG UGUGUG U	7mer-A1	1937-1943	-0.688	99.0	-0.017	36.0	noncon
5'... CCAUGAAUACACAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	1975-1982	-0.754	99.0	-0.018	37.0	noncon
5'... CACACACCAUGCAU ACACACA G... 3' AGGGAAGAAGGAGAUG UGUGUG U	7mer-A1	2008-2014	-0.683	99.0	-0.017	36.0	noncon
5'... CCAUGCAUACAUAC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	2046-2053	-0.78	99.0	-0.019	37.0	noncon
5'... ACCACACAUGCAUC CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	2135-2142	-0.743	99.0	-0.018	37.0	noncon
5'... ACCCACCACGCAUG CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	2224-2231	-0.747	99.0	-0.018	37.0	noncon
5'... CCACCACGCAUGCA CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	2226-2233	-0.798	99.0	-0.019	37.0	noncon
5'... ACAAUGCAAUCGUG CACACACA ... 3' AGGGAAGAAGGAGAU GUGUGUG U	8mer	2282-2289	-0.732	99.0	-0.018	37.0	noncon
5'... CAUGCAUACAUGCA CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	842-848	-0.407	95.0	-0.011	32.0	noncon
5'... CCAUGCAUACACAC CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	898-904	-0.398	95.0	-0.011	32.0	noncon
5'... CAUGAUCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	2196-2202	-0.406	95.0	-0.011	32.0	noncon
5'... CCAUGCAUACACAC CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1041-1047	-0.39	94.0	-0.011	32.0	noncon
5'... CCAUGCAUACACAC CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1452-1458	-0.39	94.0	-0.011	32.0	noncon
5'... ACAUACCACACAUG CACACAC G... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	2383-2389	-0.377	94.0	-0.01	32.0	noncon
5'... GCUGCACACGUAUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	821-827	-0.371	93.0	-0.01	32.0	noncon
5'... ACAUGCACACACAC CACACAC G... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	849-855	-0.359	93.0	-0.01	31.0	noncon
5'... ACAGUGCACACAUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1571-1577	-0.362	93.0	-0.01	31.0	noncon
5'... ACAGUGCACACGUG CACACAC U... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	991-997	-0.35	92.0	-0.01	31.0	noncon
5'... ACAGUGCACACGUG CACACAC U... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1134-1140	-0.348	92.0	-0.01	30.0	noncon
5'... ACCGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	917-923	-0.331	91.0	-0.009	30.0	noncon
5'... ACAAUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	2355-2361	-0.335	91.0	-0.009	30.0	noncon
5'... ACCGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1060-1066	-0.328	90.0	-0.009	30.0	noncon
5'... ACCGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1471-1477	-0.324	90.0	-0.009	30.0	noncon
5'... ACAAUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	2301-2307	-0.324	90.0	-0.009	30.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1955-1961	-0.309	89.0	-0.009	30.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1372-1378	-0.307	88.0	-0.009	30.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1402-1408	-0.304	88.0	-0.009	30.0	noncon
5'... ACAGUGCACAUGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1638-1644	-0.305	88.0	-0.009	30.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1677-1683	-0.305	88.0	-0.009	30.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	2026-2032	-0.299	88.0	-0.008	29.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	878-884	-0.291	87.0	-0.008	29.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1021-1027	-0.292	87.0	-0.008	29.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1164-1170	-0.293	87.0	-0.008	29.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1232-1238	-0.296	87.0	-0.008	29.0	noncon
5'... ACAGUGCACAUGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1335-1341	-0.292	87.0	-0.008	29.0	noncon
5'... ACAGUGCACAUGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1432-1438	-0.291	87.0	-0.008	29.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1601-1607	-0.292	87.0	-0.008	29.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1850-1856	-0.294	87.0	-0.008	29.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1994-2000	-0.296	87.0	-0.008	29.0	noncon
5'... ACAAUGCACAGGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	2245-2251	-0.283	86.0	-0.008	29.0	noncon
5'... ACAGUGCACACGUG CACACAC C... 3' AGGGAAGAAGGAGAU GUGUGUG U	7mer-m8	1747-1753	-0.269	84.0	-0.008	29.0	noncon