GlycoEnzDB

Glycogene class

Pathway figs

Glycosylation

N-linked

O-GalNAc
Glycolipid

O-Xyl

Keratan sulfates
Hyaluronan

O-GlcNAc
O-Fuc

Capping

Sugar Nucleotide

Glucuronidation

Glucuronidation

Glycan Degradation

Lysosome Degradation

Cytosol Degradation
Extracellular Degradation

Pathway Map

ALG12

General Information

Gene Symbol:

ALG12

Protein name:

Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase

Enzyme Class:

Glycosyltransferase

CAZy Family:

GT22

E.C. No:

2.4.1.260

Organs:

Reaction (SNFG):

→

P-Dol

Reaction (IUBMB):

No specified reaction.

About

• Extension of Man5GlcNAc2-P-P-Dol in the ER lumen involves the addition of four Man (by ALG3 [α(1,3)ManT], ALG9 [α(1,2)ManT], ALG12 [α(1,6)ManT] followed by ALG9 [α(1,2)ManT] again) and three Glc residues (by ALG6 [α(1,3)GlcT], ALG8 [α(1,3)GlcT] and ALG10 [α(1,2)GlcT]).
• In all instances the transfer uses either Dol-P-Man or Dol-P-Glc as donor.
• Dol-P-Man is formed from GDP-Man using enzymes DPM1,2,3
• Dol-P-Glc is formed from UDP-Glc using ALG5

Related Pathway

KEGG ORTHOLOGY:

K03847

KEGG Pathway:

ko00510 ko00513 ko01100

Reactome:

R-HSA-446193

Related Database

Organism:

Homo sapiens

Uniprot:

Q9BV10

NCBI GeneID:

79087

GlyMap GeneID:

79087

DNA aRefSeq:

NM_024105.3

Protein RefSeq:

NP_077010.1

BRENDA:

Kinetic info.

OMIM number:

607144

GeneCards database:

ALG12

Enzyme Specificity

Glycan Type:

N-linked

Compartment:

Substrate:

Man(a1-2)Man(a1-3)Man(a1-6)

Product:

Man(a1-2)Man(a1-3)[Man(a1-6)]Man(a1-6)

Constraints:

nMan=7&nMan(a1-6)=1

Simulation Parameters

Mechaelis-Menten parameters (suggested)
VM: (pmol/cell • hr) KM: (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)

ER	Cis-	Medial-	Trans-	TGN

Expression Data in Tissue {ref. 5} Source from NCBI gene database

Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database

Transcription factor {ref. 10}

miRNA-GlycoEnzyme Interactome {ref. 9}

ALG12/hsa-miR-3650

miRNA Target	Site Type	Pos.	Context Score	Frac.	Wt. Context	Frac.	Conserved
5'...CACACGUAUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	822-829	-0.626	99.0	-0.016	48.0	noncon
5'...CAUACAUGCAC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	843-850	-0.659	99.0	-0.016	49.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	879-886	-0.564	99.0	-0.014	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	892-899	-0.714	99.0	-0.018	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	918-925	-0.584	99.0	-0.015	48.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	931-938	-0.724	99.0	-0.018	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1022-1029	-0.569	99.0	-0.015	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1035-1042	-0.704	99.0	-0.017	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1061-1068	-0.579	99.0	-0.015	48.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1074-1081	-0.72	99.0	-0.018	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1165-1172	-0.571	99.0	-0.015	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1178-1185	-0.716	99.0	-0.018	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1233-1240	-0.585	99.0	-0.015	48.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1246-1253	-0.71	99.0	-0.018	50.0	noncon
5'...UGCACAUGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1336-1343	-0.573	99.0	-0.015	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1349-1356	-0.72	99.0	-0.018	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1373-1380	-0.571	99.0	-0.015	47.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1403-1410	-0.573	99.0	-0.015	47.0	noncon
5'...UGCACAUGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1433-1440	-0.563	99.0	-0.014	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1446-1453	-0.704	99.0	-0.017	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1472-1479	-0.567	99.0	-0.015	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1485-1492	-0.71	99.0	-0.018	50.0	noncon
5'...UGCACACAUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1572-1579	-0.62	99.0	-0.016	48.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1602-1609	-0.565	99.0	-0.015	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1615-1622	-0.704	99.0	-0.017	50.0	noncon
5'...UGCACAUGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1639-1646	-0.576	99.0	-0.015	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1652-1659	-0.711	99.0	-0.018	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1678-1685	-0.571	99.0	-0.015	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1691-1698	-0.71	99.0	-0.018	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1748-1755	-0.562	99.0	-0.014	47.0	noncon
5'...CACCAUGCAUG CACACCA C... 3' CCUGAGAUGUCU GUGUGG A	7mer-A1	1762-1768	-0.555	99.0	-0.014	47.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1851-1858	-0.565	99.0	-0.015	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1864-1871	-0.706	99.0	-0.017	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1956-1963	-0.592	99.0	-0.015	48.0	noncon
5'...ACACCAUGAAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1969-1976	-0.743	99.0	-0.018	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	1995-2002	-0.595	99.0	-0.015	48.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	2027-2034	-0.581	99.0	-0.015	48.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	2117-2124	-0.702	99.0	-0.017	50.0	noncon
5'...GCACAGAUGCG CACACCA U... 3' CCUGAGAUGUCU GUGUGG A	7mer-A1	2156-2162	-0.551	99.0	-0.014	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	2168-2175	-0.734	99.0	-0.018	50.0	noncon
5'...AUCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	2197-2204	-0.647	99.0	-0.016	49.0	noncon
5'...UGCACAGGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	2246-2253	-0.556	99.0	-0.014	47.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	2259-2266	-0.702	99.0	-0.017	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	2302-2309	-0.609	99.0	-0.015	48.0	noncon
5'...ACACCAUGCAU ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	2315-2322	-0.726	99.0	-0.018	50.0	noncon
5'...UGCACACGUGC ACACACCA ... 3' CCUGAGAUGUC UGUGUGG A	8mer	2356-2363	-0.615	99.0	-0.016	48.0	noncon
5'...CAAUGCACGUG CACACCA U... 3' CCUGAGAUGUCU GUGUGG A	7mer-A1	2105-2111	-0.523	98.0	-0.014	47.0	noncon
5'...GCAUACACACC ACACACC G... 3' CCUGAGAUGUC UGUGUGG A	7mer-m8	899-905	-0.321	96.0	-0.009	42.0	noncon
5'...GCAUACACACC ACACACC G... 3' CCUGAGAUGUC UGUGUGG A	7mer-m8	1042-1048	-0.32	96.0	-0.009	42.0	noncon
5'...GCAUACACACC ACACACC G... 3' CCUGAGAUGUC UGUGUGG A	7mer-m8	1453-1459	-0.315	96.0	-0.009	42.0	noncon