GlycoEnzDB
The Comprehensive Glyco-enzyme Database
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UGGT2



General Information
Gene Symbol:   
UGGT2
Protein name:   
UDP-glucose:glycoprotein glucosyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
Reaction (SNFG):   
+
+ UDP
Reaction (IUBMB):   
No specified reaction.

About

* UGGT is a large soluble protein (150-170 kDa) that is mostly ER retained.
* This is the glycoprotein folding sensor enzyme. If the glycoprotein is incompletely folded, the misfolded protein is recognized in the context of its N-glycan by the folding sensor UGGT. UGGT puts back one α(1-3)Glc, and refolding is again attempted by CNX/CRT.
* Proteins unable to properly fold after multiple cycles are eventually retro-translocated to the cytosol to be degraded by the proteasome, a process known as endoplasmic reticulum-associated degradation (ERAD).
* It is currently thought that the N-terminal may bind the misfolded polypeptide, while the C-terminal performs glycosyltransferase activity. In humans there are two proteins mediating this enzyme activity.
* Although extensively distributed in nature, UGGT is not required for single cell viability under normal conditions.


Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA_RefSeq:   
Protein_RefSeq:   
BRENDA:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     N_linked
Compartment:  
     ER
Substrate:  
     Man(a1-2)Man(a1-2)Man(a1-3)
Product:  
     Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)
Constraints:  
     nMan(a1-?)<9

Simulation Parameters
Mechaelis-Menten parameters (suggested)
Vm:       (pmol/cell • hr)       Km:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression data are collected from NCBI gene database

Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)

Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
UGGT2/hsa-miR-888-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...UAAUAAAUGACCA UUGAGUA U...
3'   ACUGACUGUCGAAA AACUCA U
7mer-A1 197-203 -0.254 97.0 -0.254 98.0 noncon