GlycoEnzDB
The Comprehensive Glyco-enzyme Database
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B3GNT8


 General Info

 About

 Related Pathway

 Related Database

 Enzyme Specificity

 Simulation Para.

 Expression Data

 Cancer Cell Line

 Transcription factor

 miRNA-GlycoEnzyme


General Information
Gene Symbol:   
B3GNT8
Protein name:   
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.*
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

* Synthesizes polylacosamine of N-glycan, particulalry along the GlcNAc(β1-6) N-glycan branch.
* B3GnT2 and B3GnT8 may form a heterocomplex to facilitate polyLacNAc extension.
* B3GNT8 is highly expressed in lung, throat, and ileum. It is further augmented during colon cancer.
* Highly metastatic cell lines may synthesize more N-glycans containing polylactosamines than poorly metastatic cell lines.

Related Pathway
KEGG ORTHOLOGY:   
K09665
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-913709
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q7Z7M8 
NCBI GeneID:   
374907
GlyMap GeneID:   
374907
DNA_RefSeq:   
NM_198540.2 
Protein_RefSeq:   
NP_940942.1 
BRENDA:   
Kinetic info.
OMIM number:   
615357
GeneCards database:   
B3GNT8
Enzyme Specificity
Glycan Type:  
     N_linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
Vm:   0.0000037    (pmol/cell • hr)       Km:   700000000    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression data are collected from NCBI gene database
Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 10 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
B3GNT8/hsa-miR-4481
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...CAUGCACUC CCCACUC U...
3'   UUGGUGGUC GGGUGAG G
 7mer-m8 217-223 -0.394 98.0 -0.292 96.0 noncon