GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
UGT8



General Information
Gene Symbol:   
UGT8
Protein name:   
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT1
E.C. No:   
2.4.1.47
Organs:   
Reaction (SNFG):   
+ Cer →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Involved in galactosylation of ceramide in ER. Reaction product galactosylceramide (GalCer) is used in synthesis of GalCer series GSLs including sulfatide.
• GalCers play an important role in myelin, since GalCers and sulfatides comprise 23 % and 4 % of the total lipid content in the myelin sheath respectively. In mice lacking this enzyme activity, myelin is unstable and degenerates resulting in the formation of large vacuoles in the ventral white matter of the spinal cord and the brain.
• CGT/UGT8-deficient mice exhibit a generalized tremor at 12-14 days followed by hind-limb paralysis, and death by 30-60 days.
• 90 % of glycolipid in the testis also consists of a unique glyceroglycolipid, seminolipid. Thus, the lipid has important functions during spermatogenesis.

Related Pathway
KEGG ORTHOLOGY:   
K04628
KEGG Pathway:   
ko00565  ko00600  ko01100 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q16880 
NCBI GeneID:   
7368
GlyMap GeneID:   
7368
DNA aRefSeq:   
NM_001128174.2 
Protein RefSeq:   
NP_001121646.1 
BRENDA:   
Kinetic info.
OMIM number:   
601291
GeneCards database:   
UGT8
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Cer
Product:  
     Gal(b1-r):Cer
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000310836 ENST00000394511
miRNA-GlycoEnzyme Interactome {ref. 9}
UGT8/hsa-miR-7109-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...UGAAAGAACACUU CCCCCCAA ...
3'   UCGUCCCAGAGGA GGGGGGU C
 8mer 548-555 -0.47 98.0 -0.407 98.0 noncon