GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
UGDH



General Information
Gene Symbol:   
UGDH
Protein name:   
UDP-glucose 6-dehydrogenase
Enzyme Class:   
CAZy Family:   
E.C. No:   
1.1.1.22
Tissues:   
all, Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   

UDP-Glc → UDP-GlcA
Reaction (IUBMB):   
No specified reaction.
About

• UDP-Glucose-6-dehydrogenase catalyzes the reaction to form UDP-glucuronic acid from UDP-glucose. Provides a pathway for UDP-GlcA to be synthesized for GAG synthesis in sugar nucleotide pathways.
• UGDH polymorphisms cause heart valve deformities during development in rare instances.
• Osteoarthritis is perpetuated by the lack of UGDH which provides additional GlcA for GAG regeneration in the synovial fluid.
• UGDH activity can be enhanced in some tumors. It causes an increase in hyaluronan synthesis, which can cause an increase in metastatic potential.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-173599
Related Database
Organism:   
Homo sapiens
Uniprot:   
O60701 
NCBI GeneID:   
7358
GlyMap GeneID:   
7358
DNA aRefSeq:   
NM_001184700.1 
Protein RefSeq:   
NP_001171629.1 
BRENDA:   
Kinetic info.
OMIM number:   
603370
GeneCards database:   
UGDH
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     
Substrate:  
     UDP-Glc
Product:  
     UDP-GlcA
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
No expression data available.
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
UGDH/hsa-miR-4755-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...GCACCCGCCACAGU GCCUGGC U...
3'   UGAAAGGGAAGUCU CGGACCG A
 7mer-m8 253-259 -0.269 93.0 -0.225 93.0 noncon
5'...GUGUGAGCCACCAC GCCUGGC C...
3'   UGAAAGGGAAGUCU CGGACCG A
 7mer-m8 387-393 -0.126 72.0 -0.106 81.0 noncon