SubPathClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
UAP1



General Information
Gene Symbol:   
UAP1
Protein name:   
UDP-N-acetylhexosamine pyrophosphorylase
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.7.7.83
Organs:   
Reaction (SNFG):   

GlcNAc(1P) or GalNAc(1P) → UDP-GlcNAc or UDP-GalNAc
Reaction (IUBMB):   
No specified reaction.
About

• Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P
• Little is known how this enzyme plays roles in disease.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-446210
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q16222 
NCBI GeneID:   
6675
GlyMap GeneID:   
6675
DNA aRefSeq:   
NM_001324113.1 
Protein RefSeq:   
NP_001311042.1 
BRENDA:   
Kinetic info.
OMIM number:   
602862
GeneCards database:   
UAP1
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     GlcNAc(1P) or GalNAc(1P)
Product:  
     UDP-GlcNAc or UDP-GalNAc
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 600 500 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-624-5p 383-390 -0.664 99.0 -0.664 99.0 noncon
hsa-miR-4470 18-25 -0.646 99.0 -0.646 99.0 noncon
hsa-miR-6890-3p 441-448 -0.611 99.0 -0.611 99.0 noncon
hsa-miR-376b-5p 99-106 -0.534 99.0 -0.534 99.0 noncon
hsa-miR-571 196-203 -0.521 99.0 -0.521 99.0 noncon
hsa-miR-549a 1229-1236 -0.508 99.0 0.0 0.0 noncon
hsa-miR-212-5p 235-242 -0.48 99.0 -0.48 99.0 con
hsa-miR-6747-3p 283-290 -0.468 99.0 -0.468 99.0 noncon
hsa-miR-376c-5p 99-106 -0.453 99.0 -0.453 99.0 noncon
hsa-miR-27a-3p 58-65 -0.441 99.0 -0.441 99.0 con
hsa-miR-27b-3p 58-65 -0.441 99.0 -0.441 99.0 con
hsa-miR-599 406-413 -0.412 99.0 -0.412 99.0 con
hsa-miR-7154-3p 669-676 -0.402 98.0 -0.001 35.0 noncon
hsa-miR-5704 825-832 -0.395 95.0 -0.001 11.0 noncon
hsa-miR-4504 1231-1238 -0.394 99.0 0.0 0.0 noncon
hsa-miR-2115-5p 316-323 -0.393 98.0 -0.393 98.0 noncon
hsa-miR-1914-5p 792-799 -0.38 98.0 -0.001 44.0 noncon
hsa-miR-4713-5p 523-530 -0.379 98.0 -0.001 19.0 noncon
hsa-miR-6736-5p 651-658 -0.363 98.0 -0.001 24.0 noncon
hsa-miR-548as-3p 107-114 -0.36 99.0 -0.36 99.0 noncon
hsa-miR-1262 651-658 -0.36 98.0 -0.001 22.0 noncon
hsa-miR-4777-5p 1189-1196 -0.347 98.0 -0.001 34.0 noncon
hsa-miR-511-5p 1009-1016 -0.345 99.0 -0.001 45.0 noncon
hsa-miR-491-3p 543-550 -0.345 96.0 -0.001 21.0 noncon
hsa-miR-4425 194-200 -0.339 95.0 -0.339 96.0 noncon
hsa-miR-3156-3p 724-731 -0.338 97.0 -0.001 32.0 noncon
hsa-miR-4718 268-274 -0.329 95.0 -0.329 97.0 noncon
hsa-miR-4701-3p 651-658 -0.328 97.0 -0.001 22.0 noncon
hsa-miR-7705 135-141 -0.324 97.0 -0.324 98.0 noncon
hsa-miR-6776-3p 1018-1024 -0.32 97.0 -0.001 41.0 noncon
hsa-miR-1255b-2-3p 736-743 -0.317 97.0 -0.001 32.0 noncon
hsa-miR-363-5p 673-680 -0.315 96.0 -0.001 17.0 noncon
hsa-miR-181b-2-3p 596-603 -0.315 98.0 -0.001 39.0 noncon
hsa-miR-181b-3p 596-603 -0.315 98.0 -0.001 39.0 noncon
hsa-miR-3688-5p 20-26 -0.309 96.0 -0.309 97.0 noncon
hsa-miR-1265 780-787 -0.307 98.0 -0.001 36.0 noncon
hsa-miR-4264 1098-1104 -0.306 97.0 -0.001 40.0 noncon
hsa-miR-4259 195-201 -0.298 94.0 -0.298 96.0 noncon
hsa-miR-1227-3p 905-911 -0.297 94.0 -0.001 31.0 noncon
hsa-miR-758-3p 1233-1239 -0.296 97.0 0.0 0.0 noncon
hsa-miR-10a-5p 104-110 -0.296 97.0 -0.296 98.0 noncon
hsa-miR-586 541-548 -0.296 97.0 -0.001 34.0 noncon
hsa-miR-10b-5p 104-110 -0.296 97.0 -0.296 98.0 noncon
hsa-miR-6731-3p 31-37 -0.296 96.0 -0.296 97.0 noncon
hsa-miR-3148 226-233 -0.295 99.0 -0.295 99.0 noncon
hsa-miR-548ao-3p 209-215 -0.291 98.0 -0.291 98.0 noncon
hsa-miR-629-3p 524-531 -0.282 98.0 -0.001 52.0 noncon
hsa-miR-6732-3p 105-111 -0.278 96.0 -0.278 97.0 noncon
hsa-miR-6745 673-680 -0.278 94.0 -0.001 14.0 noncon
hsa-miR-150-5p 522-529 -0.276 99.0 -0.001 36.0 con
hsa-miR-1295a 826-832 -0.271 93.0 -0.001 12.0 noncon
hsa-miR-4715-3p 904-910 -0.268 97.0 -0.001 44.0 noncon
hsa-miR-3609 631-638 -0.268 98.0 -0.001 45.0 noncon
hsa-miR-6854-5p 1100-1106 -0.267 97.0 -0.001 35.0 noncon
hsa-miR-3691-5p 100-106 -0.262 91.0 -0.262 93.0 noncon
hsa-miR-589-5p 1063-1069 -0.258 95.0 -0.001 34.0 noncon
hsa-miR-548ah-5p 631-638 -0.257 98.0 -0.001 43.0 noncon
hsa-miR-7155-3p 1034-1040 -0.254 94.0 -0.001 15.0 noncon
hsa-miR-3136-3p 1034-1040 -0.254 94.0 -0.001 16.0 noncon
hsa-miR-4782-5p 157-163 -0.253 96.0 -0.253 97.0 noncon
hsa-miR-4522 577-584 -0.252 94.0 -0.001 24.0 noncon
hsa-miR-181b-3p 443-449 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-181b-2-3p 443-449 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-3681-3p 58-64 -0.251 96.0 -0.251 97.0 con