GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 

Pathway Map

ST8SIA3



General Information
Gene Symbol:   
ST8SIA3
Protein name:   
Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   
+
+ CMP
Reaction (IUBMB):   
No specified reaction.

About

• ST8Sia-III transfers sialic acids to Siaα2-3Galβl-4GlcNAc sequences on N-linked oligosaccharides of glycoproteins or α2-3sialylated glycosphingolipids.
• The substrate specificity of ST8Sia-III toward glycolipids is similar to that of ST8Sia-I (GD3 synthase), and both enzymes synthesize GD3 from GM3.
• However, ST8Sia-III (and perhaps ST8Sia-5), but not ST8Sia-I, is thought to synthesize GT3 from GD3.
• The mouse ST8Sia-III gene (st8sia3) was expressed only in the brain and testis.
• Broad specificity for GSLs and proteins


Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
BRENDA:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-3)
Product:  
     NeuAc(a2-8)NeuAc(a2-3)

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000324000 MYCN RUNX1 MAX


miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-889-5p 1358-1365 -0.369 98.0 -0.369 98.0 noncon
hsa-miR-4260 41-48 -0.357 96.0 -0.357 97.0 noncon
hsa-miR-601 7437-7444 -0.355 95.0 -0.355 96.0 noncon
hsa-miR-212-5p 2246-2253 -0.321 96.0 -0.321 97.0 noncon
hsa-miR-6760-5p 463-470 -0.307 98.0 -0.307 98.0 noncon
hsa-miR-509-3p 5416-5423 -0.306 93.0 -0.306 95.0 noncon
hsa-miR-642a-3p 8133-8140 -0.303 97.0 -0.303 98.0 noncon
hsa-miR-642b-3p 8133-8140 -0.303 97.0 -0.303 98.0 noncon
hsa-miR-1825 1200-1207 -0.3 97.0 -0.3 98.0 noncon
hsa-miR-6752-3p 7322-7329 -0.298 94.0 -0.298 96.0 noncon
hsa-miR-6505-3p 7934-7941 -0.297 95.0 -0.297 96.0 noncon
hsa-miR-6804-3p 7095-7102 -0.296 94.0 -0.296 96.0 noncon
hsa-miR-3184-5p 6244-6251 -0.291 92.0 -0.291 94.0 noncon
hsa-miR-6841-5p 1030-1037 -0.29 93.0 -0.29 95.0 noncon
hsa-miR-423-5p 6244-6251 -0.283 91.0 -0.283 93.0 noncon
hsa-miR-3162-5p 464-470 -0.282 92.0 -0.282 95.0 noncon
hsa-miR-4515 708-715 -0.28 93.0 -0.28 94.0 noncon
hsa-miR-6871-5p 899-906 -0.277 95.0 -0.277 96.0 noncon
hsa-miR-5588-3p 231-238 -0.274 95.0 -0.274 96.0 noncon
hsa-miR-8071 3824-3831 -0.274 94.0 -0.274 96.0 noncon
hsa-miR-346 7339-7346 -0.27 90.0 -0.27 93.0 noncon
hsa-miR-33b-3p 3407-3414 -0.27 94.0 -0.27 96.0 noncon
hsa-miR-563 5779-5786 -0.268 92.0 -0.268 95.0 noncon
hsa-miR-4732-3p 8137-8144 -0.266 97.0 -0.266 98.0 noncon
hsa-miR-519e-3p 3407-3414 -0.259 93.0 -0.259 95.0 noncon
hsa-miR-6838-5p 239-246 -0.253 92.0 -0.253 94.0 con