GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST6GALNAC3



General Information
Gene Symbol:   
ST6GALNAC3
Protein name:   
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 3
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.7
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• Acts on some glycolipids and also O-glycans.
• ST6GalNAc-III, -V, and -VI can synthesize GD1-alpha from GM1b in gangliosides, with activity of ST6GalNAc-IV toward this substrate being poorer.
• ST6GalNAc-III exhibits relatively poor activity for GD1a [NeuAc(α2-3)Gal(β1-3)GalNAc(β1-4)[NeuAc(α2-3)]Gal(β1-4)Glc(β1-?)Cer] and GT1b [NeuAc(α2-3)Gal(β1-3)GalNAc(β1-4)[NeuAc(α2-8)NeuAc(α2-3)]Gal(β1-4)Glc(β1-?)Cer] substrates. ST6GalNAc-VI however prefers GD1a and GT1b.

Related Pathway
KEGG ORTHOLOGY:   
K03373
KEGG Pathway:   
ko00604  ko01100 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NDV1 
NCBI GeneID:   
256435
GlyMap GeneID:   
256435
DNA aRefSeq:   
NM_001160011.1 
Protein RefSeq:   
NP_001153483.1 
BRENDA:   
Kinetic info.
OMIM number:   
610133
GeneCards database:   
ST6GALNAC3
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type), GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-3)Gal(b1-3)GalNAc(b1-4)Gal(b1-4)Glc(b1-?)Cer or NeuAc(a2-3)Gal(b1-3)GalNAc(a1-?)
Product:  
     NeuAc(a2-3)Gal(b1-3)[NeuAc(a2-6)]GalNAc(b1-4)Gal(b1-4)Glc(b1-?)Cer or NeuAc(a2-3)Gal(b1-3)[NeuAc(a2-6)]GalNAc(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000328299 ENST00000464140 EGR3 SRF SP4 ERG TFAP2A AR MYC MAZ CTCF HOXB13 KLF9 MYCN NFYB NFE2 MYB TCF7L1 TFAP4 E2F4 EGR1 SMAD1 SP2 USF2 BHLHE40 E2F6 MAX TAL1 SP4 NFE2 TAF1 ZBTB14 ZBTB14
miRNA-GlycoEnzyme Interactome {ref. 9}
ST6GALNAC3/hsa-miR-1193
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'... GUGACAUGACAACU GUGACCUA ...
3'   CGUGCAGUGGCCAGA UGGUAGG G
 8mer 282-289 -0.366 98.0 -0.366 99.0 con
5'... UCCCUGCACUCCAU GUGACCU U...
3'   CGUGCAGUGGCCAGAU GGUAGG G
 6mer 4907-4913 -0.175 89.0 -0.168 92.0 noncon