GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST6GALNAC3



General Information
Gene Symbol:   
ST6GALNAC3
Protein name:   
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 3
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.7
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• Acts on some glycolipids and also O-glycans.
• ST6GalNAc-III, -V, and -VI can synthesize GD1-alpha from GM1b in gangliosides, with activity of ST6GalNAc-IV toward this substrate being poorer.
• ST6GalNAc-III exhibits relatively poor activity for GD1a [NeuAc(α2-3)Gal(β1-3)GalNAc(β1-4)[NeuAc(α2-3)]Gal(β1-4)Glc(β1-?)Cer] and GT1b [NeuAc(α2-3)Gal(β1-3)GalNAc(β1-4)[NeuAc(α2-8)NeuAc(α2-3)]Gal(β1-4)Glc(β1-?)Cer] substrates. ST6GalNAc-VI however prefers GD1a and GT1b.

Related Pathway
KEGG ORTHOLOGY:   
K03373
KEGG Pathway:   
ko00604  ko01100 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NDV1 
NCBI GeneID:   
256435
GlyMap GeneID:   
256435
DNA aRefSeq:   
NM_001160011.1 
Protein RefSeq:   
NP_001153483.1 
BRENDA:   
Kinetic info.
OMIM number:   
610133
GeneCards database:   
ST6GALNAC3
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type), GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-3)Gal(b1-3)GalNAc(b1-4)Gal(b1-4)Glc(b1-?)Cer or NeuAc(a2-3)Gal(b1-3)GalNAc(a1-?)
Product:  
     NeuAc(a2-3)Gal(b1-3)[NeuAc(a2-6)]GalNAc(b1-4)Gal(b1-4)Glc(b1-?)Cer or NeuAc(a2-3)Gal(b1-3)[NeuAc(a2-6)]GalNAc(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000328299 ENST00000464140 EGR3 SRF SP4 ERG TFAP2A AR MYC MAZ CTCF HOXB13 KLF9 MYCN NFYB NFE2 MYB TCF7L1 TFAP4 E2F4 EGR1 SMAD1 SP2 USF2 BHLHE40 E2F6 MAX TAL1 SP4 NFE2 TAF1 ZBTB14 ZBTB14
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4776-3p 5788-5795 -0.499 99.0 -0.053 72.0 noncon
hsa-miR-3115 5665-5672 -0.485 98.0 -0.464 98.0 noncon
hsa-miR-1271-5p 1407-1414 -0.428 98.0 -0.428 98.0 con
hsa-miR-4480 165-172 -0.41 98.0 -0.41 99.0 noncon
hsa-miR-3200-3p 3034-3041 -0.407 98.0 -0.39 99.0 noncon
hsa-miR-7973 2941-2948 -0.399 97.0 -0.382 97.0 noncon
hsa-miR-4455 1910-1917 -0.397 95.0 -0.397 96.0 noncon
hsa-miR-96-5p 1407-1414 -0.391 97.0 -0.391 98.0 con
hsa-miR-6876-3p 934-941 -0.386 96.0 -0.386 97.0 noncon
hsa-miR-5195-3p 245-252 -0.373 96.0 -0.373 97.0 con
hsa-miR-370-5p 282-289 -0.371 98.0 -0.371 99.0 con
hsa-miR-1183 540-547 -0.367 99.0 -0.367 99.0 noncon
hsa-miR-1193 282-289 -0.366 98.0 -0.366 99.0 con
hsa-miR-626 934-941 -0.362 96.0 -0.362 97.0 noncon
hsa-miR-3614-3p 786-793 -0.36 98.0 -0.36 98.0 noncon
hsa-miR-27a-3p 836-843 -0.355 98.0 -0.355 98.0 con
hsa-miR-27b-3p 836-843 -0.355 98.0 -0.355 98.0 con
hsa-miR-891a-5p 1645-1651 -0.345 91.0 -0.345 94.0 noncon
hsa-miR-3158-3p 3347-3354 -0.329 97.0 -0.316 98.0 noncon
hsa-miR-593-3p 2058-2065 -0.329 97.0 -0.329 98.0 noncon
hsa-miR-6747-5p 1259-1266 -0.326 96.0 -0.326 97.0 noncon
hsa-miR-6085 2213-2220 -0.324 96.0 -0.311 97.0 noncon
hsa-miR-4693-3p 126-133 -0.323 96.0 -0.323 97.0 noncon
hsa-miR-3115 1913-1920 -0.322 94.0 -0.322 96.0 noncon
hsa-miR-6813-5p 2213-2220 -0.319 96.0 -0.306 97.0 noncon
hsa-miR-943 5411-5418 -0.319 97.0 -0.306 98.0 noncon
hsa-miR-4771 4972-4979 -0.316 95.0 -0.303 96.0 noncon
hsa-miR-4745-5p 2038-2045 -0.316 94.0 -0.316 95.0 noncon
hsa-miR-8065 549-556 -0.313 93.0 -0.313 95.0 noncon
hsa-miR-4730 420-426 -0.311 91.0 -0.311 93.0 noncon
hsa-miR-4471 5622-5629 -0.307 95.0 -0.294 96.0 noncon
hsa-miR-3190-5p 3468-3475 -0.305 98.0 -0.293 98.0 noncon
hsa-miR-1251-3p 939-946 -0.304 96.0 -0.304 98.0 noncon
hsa-miR-374c-3p 1818-1825 -0.299 96.0 -0.299 97.0 noncon
hsa-miR-328-3p 4116-4123 -0.297 95.0 -0.285 96.0 noncon
hsa-miR-376a-3p 1994-2001 -0.296 95.0 -0.296 97.0 noncon
hsa-miR-376b-3p 1994-2001 -0.296 95.0 -0.296 97.0 noncon
hsa-miR-328-3p 2191-2198 -0.296 95.0 -0.284 96.0 noncon
hsa-miR-145-5p 245-252 -0.295 96.0 -0.295 97.0 con
hsa-miR-3151-5p 4949-4956 -0.294 96.0 -0.282 96.0 noncon
hsa-miR-194-5p 5581-5588 -0.293 97.0 -0.281 97.0 noncon
hsa-miR-5089-3p 660-667 -0.291 94.0 -0.291 96.0 noncon
hsa-miR-20b-5p 231-238 -0.289 97.0 -0.289 98.0 con
hsa-miR-514a-5p 5144-5151 -0.288 97.0 -0.276 98.0 noncon
hsa-miR-5590-5p 5788-5794 -0.285 96.0 -0.032 56.0 noncon
hsa-miR-7978 5141-5148 -0.285 96.0 -0.273 97.0 noncon
hsa-miR-1199-5p 5087-5094 -0.284 96.0 -0.273 97.0 noncon
hsa-miR-4520-2-3p 1197-1204 -0.283 91.0 -0.283 94.0 noncon
hsa-miR-6751-3p 5087-5094 -0.281 95.0 -0.27 96.0 noncon
hsa-miR-17-5p 231-238 -0.281 97.0 -0.281 98.0 con
hsa-miR-106a-5p 231-238 -0.278 97.0 -0.278 98.0 con
hsa-miR-6792-3p 1577-1583 -0.277 95.0 -0.277 96.0 noncon
hsa-miR-15b-3p 1717-1724 -0.275 97.0 -0.275 98.0 noncon
hsa-miR-93-5p 231-238 -0.273 97.0 -0.273 98.0 con
hsa-miR-4732-5p 1345-1351 -0.273 95.0 -0.273 97.0 noncon
hsa-miR-4314 2860-2867 -0.271 94.0 -0.26 95.0 noncon
hsa-miR-20a-5p 231-238 -0.268 97.0 -0.268 98.0 con
hsa-miR-5089-3p 3099-3106 -0.267 93.0 -0.256 95.0 noncon
hsa-miR-6888-3p 4423-4430 -0.267 96.0 -0.256 97.0 noncon
hsa-miR-3674 5516-5523 -0.267 93.0 -0.256 95.0 noncon
hsa-miR-8059 5622-5629 -0.263 95.0 -0.252 96.0 noncon
hsa-miR-6878-5p 2660-2667 -0.262 94.0 -0.252 95.0 noncon
hsa-miR-3668 5054-5060 -0.261 94.0 -0.251 95.0 noncon
hsa-miR-3614-5p 3089-3096 -0.261 97.0 -0.251 97.0 noncon
hsa-miR-4524b-5p 3493-3500 -0.26 90.0 -0.25 93.0 noncon
hsa-miR-4524a-5p 3493-3500 -0.26 90.0 -0.25 93.0 noncon
hsa-miR-106b-5p 231-238 -0.26 97.0 -0.26 98.0 con
hsa-miR-585-5p 4501-4508 -0.259 91.0 -0.249 93.0 noncon
hsa-miR-6792-3p 3206-3213 -0.257 94.0 -0.247 95.0 noncon
hsa-miR-519d-3p 231-238 -0.257 97.0 -0.257 97.0 con
hsa-miR-183-5p.1 1849-1855 -0.255 93.0 -0.255 95.0 noncon
hsa-miR-1251-5p 3484-3491 -0.255 94.0 -0.245 96.0 noncon
hsa-miR-3136-3p 653-659 -0.253 94.0 -0.253 96.0 noncon
hsa-miR-136-5p 72-79 -0.253 95.0 -0.253 97.0 noncon
hsa-miR-7106-3p 2720-2727 -0.253 92.0 -0.243 93.0 noncon
hsa-miR-6798-5p 2096-2102 -0.252 92.0 -0.242 93.0 noncon
hsa-miR-6842-3p 4272-4279 -0.251 93.0 -0.241 94.0 noncon
hsa-miR-26a-2-3p 1368-1375 -0.251 95.0 -0.251 97.0 noncon
hsa-miR-3908 3166-3173 -0.251 98.0 -0.241 98.0 noncon
hsa-miR-26a-1-3p 1368-1375 -0.251 95.0 -0.251 97.0 noncon
hsa-miR-4699-5p 5671-5678 -0.251 96.0 -0.241 97.0 noncon
hsa-miR-134-3p 2769-2776 -0.251 94.0 -0.241 95.0 noncon
hsa-miR-3137 3724-3731 -0.25 94.0 -0.24 96.0 noncon