GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 

Pathway Map

ST3GAL2



General Information
Gene Symbol:   
ST3GAL2
Protein name:   
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   
+
+ CMP
+
+ CMP
Reaction (IUBMB):   
No specified reaction.

About

• ST3Gal-II exclusively uses Type III lactosamine (Galβ1,3GalNAc) substrate. Poor activity for Type I and no activity for Type-II.
• In contrast, ST3Gal-III, ST3Gal-IV, ST3Gal-V, and ST3Gal-VI use Galβ1-3/4Glc(NAc)-R substrate
• Mainly involved in the synthesis of GD1a, GM1b and GT1b in glycolipids. Mice lacking ST3Gal-II have 50% less GD1a, GT1b.
• Mice lacking ST3Gal-II/III display complete lack of these gangliosides suggesting that these are the two dominant enzymes involved in GD1a, GT1b production.
• Can act on glycoproteins e.g. fetuin and oligosaccharide substrate, though predominant role is during glycolipid biosynthesis.
• Transgenic mice show onset of cardiac dilation and enhanced cardiomyopathy.
• Mouse and the human ST3Gal-I & -II and ST6GalNAc-I & -II contain 4 residues named Kurosawa motif (Cys-Xaa70-85-Cys-Xaα2-Cys-Ala-Val-Val-Gly-Asn-Xaa120-170-Cys; Xaa denotes any amino acid residue). These enzymes commonly utilize the Galβ1,3GalNAc structure as an acceptor substrate.
• Human ST3Gal-II mRNA is tissue specific, being prominent in skeletal muscle and heart and also expressed in liver, placenta, brain, and pancreas, while that in lung and kidney is very low. Mouse ST3Gal-II is expressed in brain, liver, and striated muscle.


Related Pathway
KEGG ORTHOLOGY:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
BRENDA:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     O-linked (mucin type), O-linked (other), GSL
Compartment:  
     
Substrate:  
     Gal(b1-3)GalNAc(b1-?) or Gal(b1-3)GlcNAc(b1-?)
Product:  
     NeuAc(a2-3)Gal(b1-3)GalNAc(b1-?) or NeuAc(a2-3)Gal(b1-3)GlcNAc(b1-?)

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    7.26e-05    (pmol/cell • hr)       KM:    2.60e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000393640 PPARG TCF12 TCF4 PPARG ZBTB17 USF2 ERG JUN AR EGR3 NR5A2


miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6781-5p 23-30 -0.745 99.0 -0.745 99.0 noncon
hsa-miR-1281 1976-1983 -0.53 98.0 -0.046 49.0 noncon
hsa-miR-6720-3p 1906-1913 -0.51 96.0 -0.044 43.0 noncon
hsa-miR-3124-5p 75-81 -0.5 91.0 -0.5 92.0 noncon
hsa-miR-4505 194-201 -0.479 99.0 -0.479 99.0 noncon
hsa-miR-1908-5p 116-123 -0.466 96.0 -0.466 97.0 noncon
hsa-miR-663a 116-123 -0.463 96.0 -0.463 97.0 noncon
hsa-miR-6787-5p 116-123 -0.461 96.0 -0.461 97.0 noncon
hsa-miR-3195 225-231 -0.459 91.0 -0.405 90.0 noncon
hsa-miR-4450 2067-2074 -0.457 98.0 -0.04 49.0 noncon
hsa-miR-3656 742-749 -0.449 97.0 -0.329 94.0 noncon
hsa-miR-6829-5p 93-100 -0.44 98.0 -0.44 99.0 noncon
hsa-miR-107 88-95 -0.439 98.0 -0.439 99.0 con
hsa-miR-103a-3p 88-95 -0.439 98.0 -0.439 99.0 con
hsa-miR-3649 2088-2095 -0.426 97.0 -0.038 50.0 noncon
hsa-miR-1225-3p 975-982 -0.425 98.0 -0.312 96.0 noncon
hsa-miR-4451 1871-1878 -0.415 97.0 -0.037 52.0 noncon
hsa-miR-6842-3p 195-202 -0.401 98.0 -0.401 99.0 noncon
hsa-miR-4707-5p 1071-1078 -0.396 96.0 -0.292 94.0 noncon
hsa-miR-5787 194-201 -0.393 99.0 -0.393 99.0 noncon
hsa-miR-6802-3p 1532-1538 -0.392 97.0 -0.245 93.0 noncon
hsa-miR-6877-5p 24-30 -0.392 95.0 -0.392 96.0 noncon
hsa-miR-6769a-5p 503-510 -0.389 98.0 -0.287 97.0 noncon
hsa-miR-6828-3p 1701-1708 -0.388 98.0 -0.035 70.0 noncon
hsa-miR-4435 1847-1854 -0.383 98.0 -0.035 65.0 noncon
hsa-miR-4704-3p 2242-2249 -0.382 98.0 -0.017 60.0 noncon
hsa-miR-6763-3p 1072-1079 -0.378 94.0 -0.279 90.0 noncon
hsa-miR-361-3p 133-140 -0.376 98.0 -0.376 98.0 noncon
hsa-miR-4460 1290-1297 -0.375 95.0 -0.277 94.0 noncon
hsa-miR-6786-5p 1586-1592 -0.364 95.0 -0.228 88.0 noncon
hsa-miR-4638-5p 1995-2002 -0.364 91.0 -0.033 52.0 noncon
hsa-miR-4743-5p 22-28 -0.361 96.0 -0.361 97.0 noncon
hsa-miR-6769b-5p 503-510 -0.347 98.0 -0.257 96.0 noncon
hsa-miR-140-5p 1289-1296 -0.344 97.0 -0.255 95.0 noncon
hsa-miR-6878-5p 190-197 -0.344 97.0 -0.344 98.0 noncon
hsa-miR-6805-5p 1302-1308 -0.337 93.0 -0.25 88.0 noncon
hsa-miR-6798-3p 959-966 -0.337 91.0 -0.25 90.0 noncon
hsa-miR-767-3p 1700-1707 -0.334 98.0 -0.031 58.0 noncon
hsa-miR-5195-5p 1530-1536 -0.332 97.0 -0.209 92.0 noncon
hsa-miR-937-5p 208-215 -0.33 98.0 -0.33 98.0 noncon
hsa-miR-509-3p 81-88 -0.325 94.0 -0.325 96.0 noncon
hsa-miR-4665-3p 638-644 -0.321 95.0 -0.238 92.0 noncon
hsa-miR-433-3p 2061-2068 -0.319 97.0 -0.029 69.0 noncon
hsa-miR-765 242-249 -0.317 95.0 -0.235 91.0 noncon
hsa-miR-6504-5p 2101-2108 -0.311 97.0 -0.029 62.0 noncon
hsa-miR-3064-5p 2101-2108 -0.303 97.0 -0.028 62.0 noncon
hsa-miR-6130 293-300 -0.302 95.0 -0.224 91.0 noncon
hsa-miR-31-5p 1618-1625 -0.301 95.0 -0.19 89.0 con
hsa-miR-5681a 2150-2156 -0.3 97.0 -0.028 63.0 noncon
hsa-miR-4283 1300-1306 -0.296 93.0 -0.22 91.0 noncon
hsa-miR-3197 759-765 -0.294 95.0 -0.219 93.0 noncon
hsa-miR-4505 2100-2106 -0.293 95.0 -0.027 53.0 noncon
hsa-miR-4430 2099-2106 -0.293 98.0 -0.027 55.0 noncon
hsa-miR-4747-5p 2146-2152 -0.289 95.0 -0.027 54.0 noncon
hsa-miR-5196-5p 2146-2152 -0.289 95.0 -0.027 54.0 noncon
hsa-miR-3652 2099-2106 -0.288 97.0 -0.027 56.0 noncon
hsa-miR-1343-5p 1331-1337 -0.286 95.0 -0.213 93.0 noncon
hsa-miR-6810-3p 1415-1422 -0.285 93.0 -0.212 91.0 noncon
hsa-miR-3150a-3p 1307-1314 -0.284 95.0 -0.211 92.0 noncon
hsa-miR-6763-5p 1307-1314 -0.284 95.0 -0.211 91.0 noncon
hsa-miR-1237-5p 570-577 -0.283 91.0 -0.211 84.0 noncon
hsa-miR-4488 570-577 -0.28 91.0 -0.208 83.0 noncon
hsa-miR-4697-5p 570-577 -0.28 90.0 -0.208 83.0 noncon
hsa-miR-4510 293-300 -0.278 93.0 -0.207 89.0 noncon
hsa-miR-6127 293-300 -0.278 93.0 -0.207 88.0 noncon
hsa-miR-6133 293-300 -0.278 93.0 -0.207 89.0 noncon
hsa-miR-6131 66-73 -0.277 97.0 -0.277 98.0 noncon
hsa-miR-5008-5p 440-447 -0.274 94.0 -0.204 92.0 noncon
hsa-miR-6745 938-945 -0.271 94.0 -0.202 90.0 noncon
hsa-miR-6129 293-300 -0.27 93.0 -0.201 89.0 noncon
hsa-miR-33a-5p 1676-1683 -0.269 99.0 -0.171 97.0 con
hsa-miR-33b-5p 1676-1683 -0.269 99.0 -0.171 97.0 con
hsa-miR-363-5p 938-945 -0.268 94.0 -0.2 90.0 noncon
hsa-miR-6503-3p 2182-2188 -0.268 95.0 -0.012 55.0 noncon
hsa-miR-939-5p 1331-1337 -0.264 93.0 -0.197 91.0 noncon
hsa-miR-6842-3p 2100-2106 -0.264 94.0 -0.025 52.0 noncon
hsa-miR-6801-3p 1415-1422 -0.263 92.0 -0.196 90.0 noncon
hsa-miR-4419a 293-300 -0.262 92.0 -0.195 88.0 noncon
hsa-miR-4707-5p 1159-1165 -0.261 90.0 -0.195 87.0 noncon
hsa-miR-3179 691-698 -0.254 93.0 -0.19 89.0 noncon
hsa-miR-3157-5p 1188-1195 -0.25 95.0 -0.187 93.0 noncon