GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST3GAL1



General Information
Gene Symbol:   
ST3GAL1
Protein name:   
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.4
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• Strict specificity for Type 3 lactosamine, predominantly on O-glycans.
• Does not add sialic acid to Galβ1,4GlcNAc (Type II).
• Although it can sialylate asialo-GM1 in vitro, mouse data suggest that it may not be acting on ganglioside substrates in vivo.
• Expressed in medullary area of thymus which is ST3Gal1+/PNA-1. In contrast cortical area is PNA+ and ST3Gal1-.
• GT-A like fold in structure, but lacking the DxD motif that binds metal ions.
• In human, ST3GAL1 is highly expressed in broadly expressed in the heart, placenta, skeletal muscle, kidney, pancreas, liver, spleen, and peripheral blood leukocytes.
• Enzyme is expressed in trans Golgi and also medial Golgi.
• ST3GAL1 deficiency in mouse decreased 90 % CD8+ T cells due to increased apoptosis, but not CD4+ T cells in the blood, lymph node, and spleen. In addition, platelet counts are decreased in ST3GAL1-deficient mice compared to wild-type mice, and platelet size is enhanced.

Related Pathway
KEGG ORTHOLOGY:   
K00780
KEGG Pathway:   
ko00512  ko00533  ko00603  ko00604  ko01100 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q11201 
NCBI GeneID:   
6482
GlyMap GeneID:   
6482
DNA aRefSeq:   
NM_003033.3 
Protein RefSeq:   
NP_003024.1 
BRENDA:   
Kinetic info.
OMIM number:   
607187
GeneCards database:   
ST3GAL1
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type), O-linked (other)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-3)...GalNAc(a1-?)Ser|Thr or Gal(b1-?)
Product:  
     NeuAc(a2-3)Gal(b1-3)...GalNAc(a1-?)Ser|Thr or NeuAc(a2-3)Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    7.26e-05    (pmol/cell • hr)       KM:    2.60e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
ST3GAL1/hsa-miR-6876-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'... GGCAGAGACAGGCA AGACAGCA ...
3'   GACUCUUCCUUUUGUG UCUGUCG A
 8mer 4341-4348 -0.377 96.0 -0.129 80.0 noncon