SubPathClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
SLC35B3



General Information
Gene Symbol:   
SLC35B3
Protein name:   
Adenosine 3'-phospho 5'-phosphosulfate transporter 2
Enzyme Class:   
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   

PAPS [Cyto] → PAPS [Golgi]
Reaction (IUBMB):   
No specified reaction.
About

• SLC35B2 and B3 transfer PAPS from the cytosol into the Golgi apparatus from the cytoplasm.
• PAPS is a universal sulfuryl donor for sulfation
• Depending on cancer type, the expression of PAPST1 and PAPST2 can increase or decrease, resulting in increases or decreases in sulfation of GAGs, respectively.
• Decreased expression of PAPST1 and PAPST2 caused FGF signaling to decrease in colorectal cells.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-174362
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9H1N7 
NCBI GeneID:   
51000
GlyMap GeneID:   
51000
DNA aRefSeq:   
NM_001142541.2 
Protein RefSeq:   
NP_001136013.1 
BRENDA:   
Kinetic info.
OMIM number:   
610845
GeneCards database:   
SLC35B3
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     PAPS [Cyto]
Product:  
     PAPS [Golgi]
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-642b-5p 33-40 -0.658 99.0 -0.658 99.0 noncon
hsa-miR-6863 201-208 -0.576 99.0 -0.576 99.0 noncon
hsa-miR-21-3p 376-383 -0.554 99.0 -0.53 99.0 noncon
hsa-miR-3591-3p 376-383 -0.519 99.0 -0.497 99.0 noncon
hsa-miR-205-5p 234-241 -0.513 99.0 -0.513 99.0 con
hsa-miR-4445-5p 37-44 -0.452 99.0 -0.452 99.0 noncon
hsa-miR-6821-5p 651-658 -0.43 98.0 -0.089 72.0 noncon
hsa-miR-2355-3p 1184-1191 -0.416 97.0 -0.086 74.0 noncon
hsa-miR-3200-3p 974-981 -0.411 98.0 -0.085 85.0 noncon
hsa-miR-371b-3p 1626-1632 -0.398 98.0 -0.083 77.0 noncon
hsa-miR-548av-3p 254-261 -0.372 99.0 -0.372 99.0 noncon
hsa-miR-431-5p 294-300 -0.365 98.0 -0.365 98.0 con
hsa-miR-328-3p 1426-1433 -0.352 97.0 -0.074 75.0 noncon
hsa-miR-6871-3p 1446-1453 -0.35 97.0 -0.074 78.0 noncon
hsa-miR-9-5p 79-85 -0.343 98.0 -0.343 99.0 con
hsa-miR-374a-3p 70-77 -0.342 99.0 -0.342 99.0 noncon
hsa-miR-4686 345-351 -0.336 97.0 -0.333 98.0 noncon
hsa-miR-3978 57-64 -0.336 99.0 -0.336 99.0 noncon
hsa-miR-2114-5p 65-71 -0.335 98.0 -0.335 98.0 noncon
hsa-miR-3657 1561-1568 -0.326 94.0 -0.069 69.0 noncon
hsa-miR-5007-3p 124-131 -0.324 98.0 -0.324 99.0 noncon
hsa-miR-6836-3p 91-97 -0.323 97.0 -0.323 98.0 noncon
hsa-miR-124-3p.2 2013-2020 -0.322 92.0 -0.068 60.0 con
hsa-miR-1279 230-237 -0.322 99.0 -0.322 99.0 noncon
hsa-miR-1255b-2-3p 1945-1952 -0.317 97.0 -0.067 81.0 noncon
hsa-miR-6822-3p 196-202 -0.316 97.0 -0.316 98.0 noncon
hsa-miR-4515 868-875 -0.311 94.0 -0.066 66.0 noncon
hsa-miR-3689c 1492-1499 -0.303 95.0 -0.065 73.0 noncon
hsa-miR-3689a-3p 1492-1499 -0.303 95.0 -0.065 73.0 noncon
hsa-miR-3689b-3p 1492-1499 -0.303 95.0 -0.065 73.0 noncon
hsa-miR-3161 2100-2107 -0.301 98.0 -0.019 66.0 noncon
hsa-miR-1915-5p 973-979 -0.299 93.0 -0.064 69.0 noncon
hsa-miR-6873-3p 508-515 -0.298 96.0 -0.064 74.0 noncon
hsa-miR-6805-3p 159-165 -0.291 97.0 -0.291 98.0 noncon
hsa-miR-5691 159-165 -0.291 96.0 -0.291 97.0 noncon
hsa-miR-1273h-5p 1492-1499 -0.287 93.0 -0.062 70.0 noncon
hsa-miR-6779-5p 1492-1499 -0.287 94.0 -0.062 71.0 noncon
hsa-miR-635 1045-1051 -0.286 95.0 -0.061 78.0 noncon
hsa-miR-4662a-5p 304-310 -0.285 96.0 -0.285 98.0 noncon
hsa-miR-4755-5p 32-38 -0.284 97.0 -0.284 98.0 noncon
hsa-miR-5006-3p 32-38 -0.284 97.0 -0.284 98.0 noncon
hsa-miR-4275 1098-1105 -0.284 98.0 -0.061 88.0 noncon
hsa-miR-3616-3p 1473-1480 -0.282 92.0 -0.06 56.0 noncon
hsa-miR-6511a-3p 1365-1372 -0.281 94.0 -0.06 71.0 noncon
hsa-miR-6511b-3p 1365-1372 -0.281 94.0 -0.06 71.0 noncon
hsa-miR-508-3p 38-44 -0.281 96.0 -0.281 97.0 noncon
hsa-miR-508-3p 1517-1524 -0.275 95.0 -0.059 65.0 noncon
hsa-miR-145-3p 1061-1068 -0.274 96.0 -0.059 77.0 noncon
hsa-miR-199a-5p 272-278 -0.273 93.0 -0.273 95.0 con
hsa-miR-199b-5p 272-278 -0.273 93.0 -0.273 95.0 con
hsa-miR-1910-5p 238-244 -0.271 94.0 -0.271 96.0 noncon
hsa-miR-30b-3p 1492-1499 -0.271 93.0 -0.058 71.0 noncon
hsa-miR-6726-5p 593-600 -0.268 91.0 -0.058 61.0 noncon
hsa-miR-4798-3p 1760-1766 -0.268 97.0 -0.058 73.0 noncon
hsa-miR-6851-3p 1425-1432 -0.266 94.0 -0.057 70.0 noncon
hsa-miR-5591-5p 1437-1443 -0.263 91.0 -0.057 61.0 noncon
hsa-miR-4300 1437-1443 -0.263 92.0 -0.057 63.0 noncon
hsa-miR-206 363-369 -0.262 94.0 -0.252 95.0 con
hsa-miR-1-3p 363-369 -0.262 94.0 -0.252 95.0 con
hsa-miR-6833-3p 432-438 -0.256 95.0 -0.221 95.0 noncon
hsa-miR-613 363-369 -0.251 93.0 -0.241 95.0 con
hsa-miR-7702 1607-1614 -0.25 93.0 -0.054 69.0 noncon
hsa-miR-6780a-5p 1492-1499 -0.25 92.0 -0.054 69.0 noncon