GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
SLC35B2



General Information
Gene Symbol:   
SLC35B2
Protein name:   
Adenosine 3'-phospho 5'-phosphosulfate transporter 1
Enzyme Class:   
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   

PAPS [Cyto] → PAPS [Golgi]
Reaction (IUBMB):   
No specified reaction.
About

• SLC35B2 and B3 transfer PAPS from the cytosol into the Golgi apparatus from the cytoplasm.
• PAPS is a universal sulfuryl donor for sulfation.
• Depending on cancer type, the expression of PAPST1 and PAPST2 can increase or decrease, resulting in increases or decreases in sulfation of GAGs, respectively.
• Decreased expression of PAPST1 and PAPST2 caused FGF signaling to decrease in colorectal cells.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-174362
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8TB61 
NCBI GeneID:   
347734
GlyMap GeneID:   
347734
DNA aRefSeq:   
NM_001286509.1 
Protein RefSeq:   
NP_001273438.1 
BRENDA:   
Kinetic info.
OMIM number:   
610788
GeneCards database:   
SLC35B2
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     PAPS [Cyto]
Product:  
     PAPS [Golgi]
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
SLC35B2/hsa-miR-4266
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...CACCACCA CCUCCUA U...
3'   CCGGUUCC GGAGGAU C
 7mer-m8 1344-1350 -0.288 93.0 -0.287 95.0 noncon