GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
SLC35A2



General Information
Gene Symbol:   
SLC35A2
Protein name:   
UDP-galactose translocator
Enzyme Class:   
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   

UDP-Gal [Cyto] → UDP-Gal[Golgi]
Reaction (IUBMB):   
No specified reaction.
About

• Multiple-segment transmembrane protein of the Golgi apparatus that delivers UDP-Gal, synthesized in the cytosol, into the Golgi lumen. This is an antiport that simultaneously exchanges luminal UMP
• This is specific for UDP-Gal and not UDP-GlcNAc. It can also transport UDP-GalNAc
• CHO-derived Lec8 are incapable of transporting UDP-Gal but are competent in UDP-GlcNAc and CMP-Sia transport
• Mutant cells accumulate Glc-Cer instead of lactosylceramide, and contain truncated N-glycans terminated at GlcNAc
• Human UDP-Gal transporter occurs in two isoforms: hUGT1 and hUGT2
• The incorporation of galactose residues into the link tetra-saccharide of proteoglycans seems to be surprisingly resistant to the deficiency of UDP-Gal, as the amounts of chondroitin sulfate and heparin sulfate were not reduced significantly in UDP-Gal transporter-deficient cells derived from MDCK cells

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-5619072
Related Database
Organism:   
Homo sapiens
Uniprot:   
P78381 
NCBI GeneID:   
7355
GlyMap GeneID:   
7355
DNA aRefSeq:   
NM_001032289.2 
Protein RefSeq:   
NP_001027460.1 
BRENDA:   
Kinetic info.
OMIM number:   
314375
GeneCards database:   
SLC35A2
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     UDP-Gal [Cyto]
Product:  
     UDP-Gal[Golgi]
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
SLC35A2/hsa-miR-558
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...ACAAAACUCCC GCAGCUC U...
3'   UAAAACCAUGU CGUCGAG U
 7mer-m8 405-411 -0.253 94.0 -0.253 96.0 noncon