GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
NEU1



General Information
Gene Symbol:   
NEU1
Protein name:   
Sialidase-1
Enzyme Class:   
Glycosidase
CAZy Family:   
GH33
E.C. No:   
3.2.1.18
Organs:   
Reaction (SNFG):   
NEU1 NEU1
H2O +
→
+
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Removes α2,3 and α2,6 sialic acid (N-acetylneuraminic acid) from glycoproteins and glycolipids in lysosomal compartments.
• Lower activity toward α2,8 sialic acid.
• During N-glycan degradation, oligosaccharides sialidase removal typically precedes activity of α- and β-Galactosidase, β-N-Acetylhexosaminidases, and α-Mannosidases. Manβ1-4GlcNAc is cleaved by β-Mannosidase in the last step.

Related Pathway
KEGG ORTHOLOGY:   
K01186
KEGG Pathway:   
ko00511  ko00600  ko04142 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q99519 
NCBI GeneID:   
4758
GlyMap GeneID:   
4758
DNA aRefSeq:   
NM_000434.3 
Protein RefSeq:   
NP_000425.1 
BRENDA:   
Kinetic info.
OMIM number:   
608272
GeneCards database:   
NEU1
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GSL
Compartment:  
     
Substrate:  
     Neu5Ac(a2-?)Gal(b1-?) or Neu5Ac(a2-?)Neu5Ac(a2-?)
Product:  
     Gal(b1-?) or Neu5Ac(a2-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
NEU1/hsa-miR-4457
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...UUUUCCUCAUGUAU CCUUGUGA ...
3'   AUGCGGUCAGUUAU GGAACAC U
 8mer 573-580 -0.352 98.0 -0.001 36.0 noncon