SubPathClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
NANS



General Information
Gene Symbol:   
NANS
Protein name:   
Sialic acid synthase
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.5.1.56
Organs:   
Reaction (SNFG):   

ManNAc(6P) or Man(6P) → Neu5Ac or Kdn
Reaction (IUBMB):   
No specified reaction.
About

• N-acetylneuraminic acid phosphate synthase reacts with mannosamine and phosphoenolpyruvate to form Neuraminic acids. It plays a crucial role in the biosynthesis of sialic acids in humans.
• NANS is essential for sialic acid development as the recycling rate of sialic acids in humans is low.
• NANS has been reported to create sialic acid KDN in some cancer contexts.
• Deficiency in NANS activity has been linked to Down?s syndrome, and delays in neurological development.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NR45 
NCBI GeneID:   
54187
GlyMap GeneID:   
54187
DNA aRefSeq:   
NM_018946.3 
Protein RefSeq:   
NP_061819.2 
BRENDA:   
Kinetic info.
OMIM number:   
605202
GeneCards database:   
NANS
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     ManNAc(6P) or Man(6P)
Product:  
     Neu5Ac or Kdn
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 200 180 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6750-5p 29-35 -0.521 99.0 0.0 44.0 noncon
hsa-miR-4471 28-34 -0.518 99.0 0.0 18.0 noncon
hsa-miR-6822-5p 29-35 -0.51 99.0 0.0 44.0 noncon
hsa-miR-8059 28-34 -0.434 99.0 0.0 26.0 noncon
hsa-miR-4251 23-29 -0.377 99.0 0.0 27.0 noncon
hsa-miR-580-3p 22-28 -0.366 99.0 0.0 36.0 noncon
hsa-miR-4756-3p 15-21 -0.325 99.0 0.0 39.0 noncon
hsa-miR-892c-5p 17-23 -0.323 98.0 0.0 21.0 noncon
hsa-miR-3919 15-21 -0.305 99.0 0.0 34.0 noncon