GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MGAT5



General Information
Gene Symbol:   
MGAT5
Protein name:   
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT18
E.C. No:   
2.4.1.155
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• MGAT5/GnT-V synthesizes the β(1,6) GlcNAc branch on α(1,6)-linked Man in N-glycans. This branching results in a product that is recognized by the lectin L-PHA.
• MGAT5 transfers to bi- and triantennary N-linked glycans that lack galactose, sialic acid and bisecting GlcNAc. The presence of galactose inhibits enzyme activity.
• Only some of native glycoproteins can serve as acceptors for GnT-V suggesting that glycan accessibility by MGAT5 is a major factor controlling glycan branching.
• Upregulation of MGAT5 is common during many cancers. It increases cell growth, decreases cell-cell adhesion and promotes tumor invasiveness.
• Note that pentaantennary N-glycans have not been reported in mammalian tissues yet. Thus GnT-VI activity due to MGAT4C may be absent.

Related Pathway
KEGG ORTHOLOGY:   
K00744
KEGG Pathway:   
ko00510  ko01100 
Reactome:   
R-HSA-975577
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q09328 
NCBI GeneID:   
4249
GlyMap GeneID:   
4249
DNA aRefSeq:   
NM_002410.4 
Protein RefSeq:   
NP_002401.1 
BRENDA:   
Kinetic info.
OMIM number:   
601774
GeneCards database:   
MGAT5
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-2)Man(a1-6)
Product:  
     GlcNAc(b1-2)[GlcNAc(b1-6)]Man(a1-6)
Constraints:  
     !GlcNAc(b1-4)...Man(b1-4)&!Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    1.00e-05    (pmol/cell • hr)       KM:    1.30e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4781-5p 2824-2831 -0.578 98.0 -0.576 99.0 noncon
hsa-miR-4730 5153-5160 -0.43 97.0 -0.411 97.0 noncon
hsa-miR-660-3p 328-335 -0.429 99.0 -0.429 99.0 noncon
hsa-miR-4749-5p 2823-2829 -0.36 93.0 -0.359 95.0 noncon
hsa-miR-4804-5p 5671-5678 -0.359 90.0 -0.344 92.0 noncon
hsa-miR-608 1297-1304 -0.345 91.0 -0.344 92.0 noncon
hsa-miR-4651 1297-1304 -0.332 90.0 -0.331 92.0 noncon
hsa-miR-483-3p.1 2957-2964 -0.329 98.0 -0.328 98.0 noncon
hsa-miR-4706 2823-2829 -0.328 90.0 -0.327 92.0 noncon
hsa-miR-4435 3818-3825 -0.323 96.0 -0.31 97.0 noncon
hsa-miR-3670 4290-4297 -0.311 95.0 -0.298 96.0 noncon
hsa-miR-634 2981-2988 -0.309 96.0 -0.308 98.0 noncon
hsa-miR-124-3p.1 3164-3170 -0.3 95.0 -0.288 96.0 con
hsa-miR-377-3p 5768-5774 -0.295 96.0 -0.001 22.0 con
hsa-miR-155-3p 4362-4369 -0.282 93.0 -0.27 94.0 noncon
hsa-miR-4664-5p 3578-3584 -0.269 93.0 -0.258 94.0 noncon
hsa-miR-6748-3p 953-960 -0.268 93.0 -0.267 95.0 noncon
hsa-miR-4727-5p 3490-3496 -0.268 95.0 -0.257 96.0 noncon
hsa-miR-365a-3p 2554-2561 -0.26 93.0 -0.259 96.0 con
hsa-miR-365b-3p 2554-2561 -0.26 93.0 -0.259 96.0 con
hsa-miR-342-5p 3578-3584 -0.259 92.0 -0.249 93.0 noncon
hsa-miR-4727-5p 1905-1911 -0.256 94.0 -0.255 96.0 noncon