GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MGAT3



General Information
Gene Symbol:   
MGAT3
Protein name:   
Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT17
E.C. No:   
2.4.1.144
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• MGAT3 or GnT-III transfers β1,4-linked GlcNAc to the α1,4-linked Man in N-glycans. The resulting structure is termed 'bisecting GlcNAc', and plays a regulatory role(s) in the biosynthesis of complex and hybrid N-glycans.
• Prior MGAT1 activity is necessary for MGAT3 action. Previous βGal addition inhibits future MGAT3 activity. Prior α(1-6)fucosylation does not affect MGAT3, but bisecting structures will hinder core-fucosylation.
• Addition of MGAT3 also terminates N-glycan branching by inhibiting other GlcNAcTs.
• Overexpression of MGAT3 prevents N-glycan branching, reduces metastatic potential, lowers galectin lattice formation and inhibits epithelia-mesenchymal transition (EMT) induced by TGF-β1.

Related Pathway
KEGG ORTHOLOGY:   
K00737
KEGG Pathway:   
ko00510  ko01100 
Reactome:   
R-HSA-975574
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q09327 
NCBI GeneID:   
4248
GlyMap GeneID:   
4248
DNA aRefSeq:   
NM_001098270.1 
Protein RefSeq:   
NP_001091740.1 
BRENDA:   
Kinetic info.
OMIM number:   
604621
GeneCards database:   
MGAT3
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     ...Man(b1-4)
Product:  
     ...[GlcNAc(b1-4)]Man(b1-4)
Constraints:  
     nGlcNAc(b1-2)Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(b1-r)>=1&!Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    1.00e-04    (pmol/cell • hr)       KM:    4.00e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000341184 CTCF NFE2 TCF7L1 YY1 CTCFL SP1 BHLHE40 EGR3 TAL1 GRHL3 NRF1 EGR2 TFAP2A EGR1 PAX5 TEAD4 KLF1 GATAD2B MYNN MXI1 CTCF CRY1 POU5F1
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4532 2880-2887 -0.573 98.0 -0.573 99.0 noncon
hsa-miR-219b-5p 3090-3097 -0.453 98.0 -0.453 98.0 noncon
hsa-miR-499b-5p 3237-3244 -0.437 99.0 -0.233 96.0 noncon
hsa-miR-541-3p 384-391 -0.38 97.0 -0.38 98.0 noncon
hsa-miR-6775-3p 2796-2803 -0.379 96.0 -0.379 97.0 noncon
hsa-miR-654-5p 384-391 -0.364 97.0 -0.364 98.0 noncon
hsa-miR-3918 2894-2901 -0.36 97.0 -0.36 98.0 noncon
hsa-miR-193b-3p 1035-1041 -0.35 95.0 -0.35 97.0 noncon
hsa-miR-193a-3p 1035-1041 -0.35 95.0 -0.35 97.0 noncon
hsa-miR-324-5p 3191-3197 -0.329 94.0 -0.329 96.0 con
hsa-miR-6798-3p 124-130 -0.324 90.0 -0.324 94.0 noncon
hsa-miR-6724-5p 1014-1021 -0.3 95.0 -0.3 96.0 noncon
hsa-miR-6862-5p 2475-2482 -0.294 90.0 -0.294 93.0 noncon
hsa-miR-3162-3p 127-133 -0.291 91.0 -0.291 94.0 noncon
hsa-miR-1291 2796-2803 -0.289 93.0 -0.289 94.0 noncon
hsa-miR-6773-5p 1014-1021 -0.279 92.0 -0.279 95.0 noncon
hsa-miR-1915-3p 3068-3075 -0.275 97.0 -0.275 98.0 noncon
hsa-miR-4279 1491-1498 -0.271 96.0 -0.271 97.0 noncon
hsa-miR-4279 846-853 -0.269 96.0 -0.269 97.0 noncon
hsa-miR-6127 2804-2811 -0.26 91.0 -0.26 93.0 noncon
hsa-miR-6133 2804-2811 -0.26 92.0 -0.26 94.0 noncon