GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MAN2A1



General Information
Gene Symbol:   
MAN2A1
Protein name:   
Alpha-mannosidase 2
Enzyme Class:   
Glycosidase
CAZy Family:   
GH38
E.C. No:   
3.2.1.114
Organs:   
Reaction (SNFG):   
MAN2A1 MAN2A1
H2O +
→
+
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Golgi resident mannosidase that cleaves the final two α1,3-Man and α1,6-Man during N-glycan processing.
• Man2α2 and Man2a1 act as isozymes, with similar α1,3/6-Man specificity.
• Absence of both is necessary for depeletion of complex N-glycans in mice, suggesting functional redundancy.
• Mice lacking MAN2A1 exhibit many of the features of humans lacking this enzyme activity including significant dyserythropoiesis, anemia, splenomegaly, and immature erythrocytes or reticulocytes in the peripheral blood, but no neurologic or behavioral symptoms, no reproductive problems, and no major histological findings in most tissues.
• Man2α2-deficient male mice are largely inferile, but females are normal.
• Swainsonine is a α-Mannosidase inhibitor.

Related Pathway
KEGG ORTHOLOGY:   
K01231
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-6811438
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q16706 
NCBI GeneID:   
4124
GlyMap GeneID:   
4124
DNA aRefSeq:   
NM_002372.3 
Protein RefSeq:   
NP_002363.2 
BRENDA:   
Kinetic info.
OMIM number:   
154582
GeneCards database:   
MAN2A1
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Man(a1-6)...Man(a1-6) or Man(a1-3)...Man(a1-6)
Product:  
     ...Man(a1-6) or ...Man(a1-6)
Constraints:  
     !Gal(b1-?)&nGlcNAc(b1-2)Man(a1-3)>=1&nGlcNAc(b1-?)=3
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.00e-04    (pmol/cell • hr)       KM:    2.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000261483
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-124-3p.1 834-841 -0.462 99.0 -0.441 99.0 con
hsa-miR-3119 177-184 -0.416 98.0 -0.409 98.0 noncon
hsa-miR-4695-3p 1828-1835 -0.395 98.0 -0.366 98.0 noncon
hsa-miR-2682-3p 643-650 -0.358 98.0 -0.342 98.0 noncon
hsa-miR-367-3p 59-66 -0.353 97.0 -0.353 98.0 con
hsa-miR-363-3p 59-66 -0.353 97.0 -0.353 98.0 con
hsa-miR-25-3p 59-66 -0.34 97.0 -0.34 98.0 con
hsa-miR-452-3p 1931-1938 -0.336 98.0 -0.312 98.0 noncon
hsa-miR-124-3p.2 835-841 -0.336 93.0 -0.321 94.0 con
hsa-miR-32-5p 59-66 -0.327 97.0 -0.327 97.0 con
hsa-miR-92b-3p 59-66 -0.319 97.0 -0.319 97.0 con
hsa-miR-92a-3p 59-66 -0.319 97.0 -0.319 97.0 con
hsa-miR-3157-5p 400-407 -0.316 97.0 -0.311 98.0 noncon
hsa-miR-1343-3p 1820-1827 -0.314 97.0 -0.292 97.0 noncon
hsa-miR-6781-3p 643-650 -0.313 97.0 -0.299 98.0 noncon
hsa-miR-6783-3p 1820-1827 -0.301 96.0 -0.28 97.0 noncon
hsa-miR-6878-5p 217-224 -0.3 96.0 -0.295 97.0 noncon
hsa-miR-1263 723-729 -0.295 92.0 -0.282 94.0 noncon
hsa-miR-3187-3p 438-445 -0.283 98.0 -0.271 98.0 noncon
hsa-miR-506-3p 835-841 -0.274 92.0 -0.262 94.0 con
hsa-miR-670-5p 2123-2130 -0.266 98.0 -0.248 98.0 noncon
hsa-miR-16-1-3p 2382-2389 -0.264 97.0 -0.246 97.0 noncon
hsa-miR-135b-5p 179-185 -0.263 93.0 -0.259 95.0 con
hsa-miR-135a-5p 179-185 -0.263 93.0 -0.259 95.0 con
hsa-miR-5582-5p 833-839 -0.258 94.0 -0.247 95.0 noncon
hsa-miR-3938 2580-2586 -0.256 94.0 -0.238 95.0 noncon
hsa-miR-133a-5p 2079-2085 -0.251 92.0 -0.234 93.0 noncon