GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS6ST1



General Information
Gene Symbol:   
HS6ST1
Protein name:   
Heparan-sulfate 6-O-sulfotransferase 1
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Three heparan sulfate 6-O-sulfotransferases exist that transfer sulfate to the C6 position of GlcNAc or GlcNS in heparan sulfate/heparin.
• HS6ST1 prefers iduronic acid residues adjacent to the GlcNS. HS6ST2 prefers GlcA when substrate conc is high, but it prefers IdoA at low substrate concentration. HS6ST-3 preference is intermediate between HS6ST1 and HS6ST2. These enzymes do not function when IdoA is 2-sulfated.
• HS6ST1 is ubiquitously expressed. The expression of other isoenzymes is restricted. HS6ST2 is expressed primarily in bladder, brain, testis, and thyroid. HS6ST3 slightly in brain, connective tissue, and kidney.
• HS6ST1 deficient mice display growth retardation and fatality after E15.5. H6ST1 is also associated with human disease.
• These enzymes do not act on CS/DS/KS.

Related Pathway
KEGG ORTHOLOGY:   
K02514
KEGG Pathway:   
ko00534 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
O60243 
NCBI GeneID:   
9394
GlyMap GeneID:   
9394
DNA aRefSeq:   
NM_004807.2 
Protein RefSeq:   
NP_004798.3 
BRENDA:   
Kinetic info.
OMIM number:   
604846
GeneCards database:   
HS6ST1
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4)GlcN(a1-4 [2S])
Product:  
     IdoA(a1-4)GlcN(a1-4 [2,6S])
Constraints:  
     ! < IdoA(a1-4 [2S])>
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
HS6ST1/hsa-miR-4508
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...GCCCCCAAU GCCCCGA G...
3'   GCGCGCGGGU CGGGGC G
 7mer-A1 503-509 -0.259 91.0 -0.259 93.0 noncon