GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GMPPA



General Information
Gene Symbol:   
GMPPA
Protein name:   
Mannose-1-phosphate guanyltransferase alpha
Enzyme Class:   
N_linked
CAZy Family:   
E.C. No:   
Tissues:   
all, Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   

Man(1P) → GDP-Man
Reaction (IUBMB):   
No specified reaction.
About

• Synthesizes GDP-mannose from mannose 1 phosphate and GTP.
• Knowledge of GMPPA and B is subsumed based on porcupine enzyme activity and the homology of porcupine and GMPPA and B structures.
• GDP-mannose is a critical reactant for sugar nucleotide synthesis and glycosylation, as it can be directly used in the synthesis of N-linked glycans and can be metabolized into other sugar nucleotide donors.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-446205
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q96IJ6 
NCBI GeneID:   
29926
GlyMap GeneID:   
29926
DNA aRefSeq:   
NM_013335.3 
Protein RefSeq:   
NP_037467.2 
BRENDA:   
Kinetic info.
OMIM number:   
615495
GeneCards database:   
GMPPA
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     Man(1P)
Product:  
     GDP-Man
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4707-5p 112-119 -0.729 99.0 -0.729 99.0 noncon
hsa-miR-5580-5p 191-198 -0.68 99.0 0.0 11.0 noncon
hsa-miR-3162-5p 164-171 -0.651 99.0 -0.65 99.0 noncon
hsa-miR-7152-3p 80-87 -0.61 99.0 -0.61 99.0 noncon
hsa-miR-28-5p 23-30 -0.489 99.0 -0.489 99.0 noncon
hsa-miR-708-5p 23-30 -0.489 99.0 -0.489 99.0 noncon
hsa-miR-3139 23-30 -0.455 99.0 -0.455 99.0 noncon
hsa-miR-3191-5p 83-90 -0.452 99.0 -0.452 99.0 noncon
hsa-miR-6849-3p 95-102 -0.449 99.0 -0.449 99.0 noncon
hsa-miR-6764-3p 82-89 -0.438 99.0 -0.438 99.0 noncon
hsa-miR-6824-3p 82-89 -0.414 99.0 -0.414 99.0 noncon
hsa-miR-149-5p 190-196 -0.378 98.0 0.0 0.0 con
hsa-miR-4745-5p 31-37 -0.353 96.0 -0.353 97.0 noncon
hsa-miR-6771-5p 157-163 -0.351 92.0 -0.35 95.0 noncon
hsa-miR-6763-3p 113-119 -0.347 92.0 -0.347 94.0 noncon
hsa-miR-4638-3p 70-77 -0.346 97.0 -0.346 97.0 noncon
hsa-miR-6746-5p 157-163 -0.342 96.0 -0.341 97.0 noncon
hsa-miR-193b-5p 173-179 -0.329 93.0 -0.329 95.0 noncon
hsa-miR-1229-5p 28-34 -0.315 97.0 -0.315 98.0 noncon
hsa-miR-4529-5p 128-134 -0.311 93.0 -0.311 95.0 noncon
hsa-miR-4488 17-23 -0.309 93.0 -0.309 94.0 noncon
hsa-miR-629-5p 172-178 -0.309 98.0 -0.308 98.0 noncon
hsa-miR-6512-3p 96-102 -0.307 97.0 -0.307 97.0 noncon
hsa-miR-5587-3p 113-119 -0.303 92.0 -0.303 94.0 noncon
hsa-miR-6760-5p 164-170 -0.3 97.0 -0.299 98.0 noncon
hsa-miR-4728-5p 155-161 -0.299 96.0 -0.299 96.0 noncon
hsa-miR-4697-5p 17-23 -0.297 92.0 -0.297 94.0 noncon
hsa-miR-6720-5p 96-102 -0.296 96.0 -0.296 97.0 noncon
hsa-miR-1237-5p 17-23 -0.293 92.0 -0.293 94.0 noncon
hsa-miR-4481 31-37 -0.291 96.0 -0.291 96.0 noncon
hsa-miR-4731-5p 19-25 -0.289 96.0 -0.289 97.0 noncon
hsa-miR-637 18-24 -0.286 95.0 -0.286 96.0 noncon
hsa-miR-4739 164-170 -0.278 95.0 -0.278 96.0 noncon
hsa-miR-6785-5p 155-161 -0.277 95.0 -0.277 96.0 noncon
hsa-miR-6883-5p 155-161 -0.267 94.0 -0.267 95.0 noncon
hsa-miR-1289 138-144 -0.262 91.0 -0.262 93.0 noncon
hsa-miR-4487 21-27 -0.254 93.0 -0.254 95.0 noncon
hsa-miR-6127 153-159 -0.251 90.0 -0.251 93.0 noncon
hsa-miR-5586-5p 97-103 -0.25 94.0 -0.25 96.0 noncon