GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT9



General Information
Gene Symbol:   
FUT9
Protein name:   
Alpha-(1,3)-fucosyltransferase 9
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT10
E.C. No:   
2.4.1.152
Organs:   
Reaction (SNFG):   
+
→
+ GDP
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• FUT9 is restricted to subpopulations such as granulocytes, natural killer cells, and B cells. It is also highly expressed in the brain.
• In blood cells, it forms the LewisX/CD15/SSEA-1 antigen and it also helps form LeY structures.
• May participate in forming E-selectin ligand in humans, along with FUT4 and 7.

Related Pathway
KEGG ORTHOLOGY:   
K03663
KEGG Pathway:   
ko00515  ko00601  ko00603  ko01100 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y231 
NCBI GeneID:   
10690
GlyMap GeneID:   
10690
DNA aRefSeq:   
NM_006581.3 
Protein RefSeq:   
NP_006572.2 
BRENDA:   
Kinetic info.
OMIM number:   
606865
GeneCards database:   
FUT9
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?) or ...GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-?)
Product:  
     Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?) or ...GlcNAc(b1-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.75e-06    (pmol/cell • hr)       KM:    1.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
FUT9/hsa-miR-218-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AUAGGGUUAUUUU AAGCACA C...
3'   UGUACCAAUCUAG UUCGUGU U
 7mer-m8 9433-9439 -0.308 95.0 -0.308 97.0 con
5'...GAAUCUAAAGGAU AAGCACA G...
3'   UGUACCAAUCUAG UUCGUGU U
 7mer-m8 4215-4221 -0.065 66.0 -0.065 75.0 noncon