GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT4



General Information
Gene Symbol:   
FUT4
Protein name:   
Alpha-(1,3)-fucosyltransferase 4
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT10
E.C. No:   
2.4.1.152
Organs:   
Reaction (SNFG):   
+
→
+ GDP
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• Although FUT4 is ubiquitously expressed in almost all tissues, it is most abundant in myeloid cells (granulocytes, monocytes) and also lymphocytes.
• Prefers to act on inner GlcNAc of polylactosamine chains of neutral and sialylated polylactosamine in blood cells, but can also form the LeX structure in the absence of FUT9.
• While FUT4, 7 and 9 are expressed on blood cells, FUT3, FUT5, and FUT6 are not.
• FUT4 mainly participates in VIM-2 (CDw65) formation.
• FUT4 plays a role in some aspects of leukocyte rolling, but is not the dominant enzyme regulating this process.

Related Pathway
KEGG ORTHOLOGY:   
K07632
KEGG Pathway:   
ko00515  ko00601  ko01100 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
P22083 
NCBI GeneID:   
2526
GlyMap GeneID:   
2526
DNA aRefSeq:   
NM_002033.3 
Protein RefSeq:   
NP_002024.1 
BRENDA:   
Kinetic info.
OMIM number:   
104230
GeneCards database:   
FUT4
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?) or NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-?)
Product:  
     Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?) or NeuAc(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?)
Constraints:  
     !GlcNAc(b1-6)[GlcNAc(b1-3)]Gal(b1-4)@GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.75e-06    (pmol/cell • hr)       KM:    1.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
No expression data available.
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6820-5p 77-84 -0.618 98.0 -0.618 98.0 noncon
hsa-miR-609 105-112 -0.49 98.0 -0.49 98.0 noncon
hsa-miR-365b-5p 711-718 -0.477 99.0 -0.477 99.0 noncon
hsa-miR-365a-5p 711-718 -0.461 99.0 -0.461 99.0 noncon
hsa-miR-3913-5p 713-720 -0.427 99.0 -0.427 99.0 noncon
hsa-miR-6802-3p 243-250 -0.401 97.0 -0.401 98.0 noncon
hsa-miR-6888-5p 794-801 -0.385 99.0 -0.385 99.0 noncon
hsa-miR-4290 541-548 -0.382 95.0 -0.382 96.0 noncon
hsa-miR-125a-5p 4070-4077 -0.376 97.0 -0.009 40.0 con
hsa-miR-4319 4070-4077 -0.376 97.0 -0.009 40.0 con
hsa-miR-8077 154-161 -0.368 99.0 -0.368 99.0 noncon
hsa-miR-125b-5p 4070-4077 -0.368 97.0 -0.009 39.0 con
hsa-miR-425-3p 396-402 -0.362 94.0 -0.362 96.0 noncon
hsa-miR-4716-3p 3324-3331 -0.356 95.0 -0.009 19.0 noncon
hsa-miR-3177-5p 4117-4124 -0.355 94.0 -0.009 23.0 noncon
hsa-miR-1301-3p 696-702 -0.354 97.0 -0.354 98.0 noncon
hsa-miR-3122 713-720 -0.351 99.0 -0.351 99.0 noncon
hsa-miR-3684 2268-2275 -0.346 95.0 -0.009 28.0 noncon
hsa-miR-6794-5p 3324-3331 -0.343 95.0 -0.009 20.0 noncon
hsa-miR-1304-5p 3971-3978 -0.341 97.0 -0.008 44.0 noncon
hsa-miR-23b-3p 781-788 -0.335 96.0 -0.335 96.0 con
hsa-miR-23a-3p 781-788 -0.335 96.0 -0.335 96.0 con
hsa-miR-2115-5p 607-614 -0.334 97.0 -0.334 97.0 noncon
hsa-miR-5047 696-702 -0.321 97.0 -0.321 98.0 noncon
hsa-miR-5008-3p 158-165 -0.32 97.0 -0.32 97.0 noncon
hsa-miR-6827-5p 209-215 -0.314 93.0 -0.314 94.0 noncon
hsa-miR-6735-3p 347-354 -0.313 97.0 -0.313 98.0 noncon
hsa-miR-4793-3p 279-286 -0.311 98.0 -0.311 98.0 noncon
hsa-miR-4517 850-857 -0.31 99.0 -0.31 99.0 noncon
hsa-miR-7157-3p 158-165 -0.307 96.0 -0.307 97.0 noncon
hsa-miR-6792-3p 632-639 -0.306 96.0 -0.306 97.0 noncon
hsa-miR-4755-3p 3539-3546 -0.305 95.0 -0.008 41.0 noncon
hsa-miR-6079 372-379 -0.303 97.0 -0.303 98.0 noncon
hsa-miR-6737-3p 158-165 -0.299 96.0 -0.299 97.0 noncon
hsa-miR-23c 781-788 -0.295 95.0 -0.295 96.0 con
hsa-miR-765 1621-1628 -0.293 94.0 -0.007 34.0 noncon
hsa-miR-3064-3p 2890-2897 -0.281 93.0 -0.007 34.0 noncon
hsa-miR-135b-3p 3327-3334 -0.28 90.0 -0.007 29.0 noncon
hsa-miR-1302 713-719 -0.276 94.0 -0.276 96.0 noncon
hsa-miR-4298 713-719 -0.273 94.0 -0.273 96.0 noncon
hsa-miR-6878-3p 30-37 -0.272 94.0 -0.272 96.0 noncon
hsa-miR-6772-5p 106-112 -0.268 93.0 -0.268 95.0 noncon
hsa-miR-4724-5p 738-745 -0.264 98.0 -0.264 98.0 noncon
hsa-miR-1294 663-669 -0.264 94.0 -0.264 96.0 noncon
hsa-miR-130a-5p 781-788 -0.261 94.0 -0.261 95.0 con
hsa-miR-4717-5p 3253-3259 -0.26 95.0 -0.007 50.0 noncon
hsa-miR-19a-3p 190-197 -0.257 91.0 -0.257 93.0 con
hsa-miR-5586-5p 619-625 -0.255 94.0 -0.255 96.0 noncon
hsa-miR-1288-3p 546-553 -0.255 91.0 -0.255 94.0 noncon