GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT1



General Information
Gene Symbol:   
FUT1
Protein name:   
Galactoside 2-alpha-L-fucosyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT11
E.C. No:   
2.4.1.344
Organs:   
Reaction (SNFG):   
+
→
+ GDP
+
→
+ GDP
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• FUT1 and FUT2 α2-fucosyltransferases are encoded by the H and Se genes, respectively. Sec1 is a related pseudogene that has no functon in humans.
• FUT1 and FUT2 transfer fucose in α(1,2) linkage onto the terminal galactose of lactosamine predominantly on type 1 (Galβ1,3GlcNAcβ1-R) and type 2 (Galβ1,4GlcNAcβ1-R) chains. However, these enzymes can also work on type 3 (Galβ1,3GalNAcα1-R), type 4 (Galβ1,3GalNAcβ1-R), type 5 (Galβ1,3Galβ1-R), or type 6 (Galβ1,4Glcβ1-R) disaccharide acceptors although less efficiently.
• Final products of α2-fucosyltransferases are: CD173 or monofucosylated H type 2 (Fucα1,2Galβ1,4GlcNAcβ1-R) & CD174 or difucosylated Ley (Fucα1,2Galβ1,4[Fucα1,3]GlcNAcβ1-R).
• FUT1 (H) is expressed predominantly in erythroid tissues and primary sensory neurons (cells originating in the ecto- and meso-derm).
• Tissue specific distribution of a(1-2)FUT activity in rodent models is quite different from humans.
• The fucosylated glycan products of FUT1 and FUT2 may serve as ligands in cell adhesion or as receptors for certain microorganisms and play a role in susceptibility to infection.

Related Pathway
KEGG ORTHOLOGY:   
K00718
KEGG Pathway:   
ko00601  ko00603  ko01100 
Reactome:   
R-HSA-9033807
Related Database
Organism:   
Homo sapiens
Uniprot:   
P19526 
NCBI GeneID:   
2523
GlyMap GeneID:   
2523
DNA aRefSeq:   
NM_000148.3 
Protein RefSeq:   
NP_000139.1 
BRENDA:   
Kinetic info.
OMIM number:   
211100
GeneCards database:   
FUT1
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-?)...Gal(b1-?) or Gal(b1-?)GalNAc(b1-?) or Gal(b1-?)GlcNAc(b1-?)
Product:  
     Fuc(a1-2)Gal(b1-?)...Gal(b1-?) or Fuc(a1-2)Gal(b1-?)GalNAc(b1-?) or Fuc(a1-2)Gal(b1-?)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.75e-06    (pmol/cell • hr)       KM:    1.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
FUT1/hsa-miR-4473
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AAUUAAAAGUCUAC AGCACUAA ...
3'   AUGAACAUUGCCUC UCGUGAU C
 8mer 2166-2173 -0.531 98.0 -0.138 78.0 noncon