FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUCA1



General Information
Gene Symbol:   
FUCA1
Protein name:   
Tissue alpha-L-fucosidase
Enzyme Class:   
Glycosidase
CAZy Family:   
GH29
E.C. No:   
3.2.1.51
Organs:   
Reaction (SNFG):   
FUCA1
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• L-α-fucosidase in humans, that cleaves α1-6 linked fucose from GlcNAc on glycoproteins.
• It may also degrade other aspects of glycoprotein and glycolipid fucosylation.
• FUCA1 mutations are associated with fucosidosis (FUCA1D), an autosomal recessive lysosomal storage disease. These patients have intact N-glycans attached to Asn, suggesting that fucosidase activity is one of the first steps during N-glycan degradation.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-6798695
Related Database
Organism:   
Homo sapiens
Uniprot:   
P04066 
NCBI GeneID:   
2517
GlyMap GeneID:   
2517
DNA aRefSeq:   
NM_000147.4 
Protein RefSeq:   
NP_000138.2 
BRENDA:   
Kinetic info.
OMIM number:   
612280
GeneCards database:   
FUCA1
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (other)
Compartment:  
     
Substrate:  
     Fuc(a1-6)GlcNAc(b1-?)Asn
Product:  
     GlcNAc(b1-?)Asn
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000374479 PPARG NFE2 SPIB CEBPB MYB PPARG CTCF SPIB ESR1 MAX NCOR1 TCF12 IKZF1 FOXA2 RUNX1 IKZF1 TEAD1 E2F1 ASCL1 MAX NFE2 CTCF CTCFL YY1 STAT3 ZNF770 STAT5B TEAD2 PPARG ZFHX3 ARNT2 AR MITF GRHL1 MYC ZNF83 VEZF1 CTCFL MAFK E2F1 CEBPD ZBTB33 ZNF3 RUNX1 NR5A2 DNMT3B MAX MAZ STAT2 KLF4 KLF4 BATF DMAP1 TGIF2 KLF15 KLF15 PKNOX1 ZNF652 PBX3 PBX3 ZBTB2 TAF1 TFE3 PPARG KMT2B ZBTB48 ZBTB48 TCF4 TCF4 ETV6 HOMEZ ELF4 ELF4 RCOR1 EBF1 USF2 USF2 USF2 MECOM THAP11 ZNF444 USF1 USF1 USF1 USF1 HOXA4 HOXA4 NFE2 RFX5 BCL11B BCL11B PAX5 ARID4B ASCL1 ZNF511 JUN IRF3 ID3 NR2C2 SP4 SP4 MIER3 KLF1 E2F4 ZSCAN9 ZSCAN9 FOS FOS FOS SP5 SP5 SCRT1 ZNF35 BHLHE40 MAF FOXP1 KLF9 KLF9 KLF9 TCF12 ZGPAT E2F6 EGR3 EGR1 STAT5B NFATC1 ATF2 TFAP4 ZBTB26 RFXANK EGR2 NR4A1 KLF6 CREM ZBTB14 ZBTB14 GLIS1 PAX5 MAX MAX ETS1 TCF3 ZNF580 NONO
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4650-3p 625-632 -0.664 99.0 -0.015 30.0 noncon
hsa-miR-133a-3p.2 167-174 -0.501 98.0 -0.501 98.0 noncon
hsa-miR-133b 167-174 -0.501 98.0 -0.501 98.0 noncon
hsa-miR-584-3p 136-143 -0.489 99.0 -0.489 99.0 noncon
hsa-miR-1-5p 587-594 -0.468 99.0 -0.468 99.0 noncon
hsa-miR-363-5p 97-104 -0.464 99.0 -0.464 99.0 noncon
hsa-miR-5691 504-511 -0.463 99.0 -0.463 99.0 noncon
hsa-miR-6805-3p 504-511 -0.455 99.0 -0.455 99.0 noncon
hsa-miR-6745 97-104 -0.451 99.0 -0.451 99.0 noncon
hsa-miR-668-5p 22-28 -0.451 96.0 -0.451 97.0 noncon
hsa-miR-3679-3p 451-458 -0.416 97.0 -0.416 98.0 noncon
hsa-miR-3935 271-278 -0.409 98.0 -0.409 99.0 noncon
hsa-miR-326 101-108 -0.393 98.0 -0.393 98.0 con
hsa-miR-5703 93-100 -0.377 99.0 -0.377 99.0 noncon
hsa-miR-6776-5p 99-106 -0.375 98.0 -0.375 99.0 noncon
hsa-miR-330-5p 101-108 -0.372 98.0 -0.372 98.0 con
hsa-miR-4434 93-100 -0.369 98.0 -0.369 99.0 noncon
hsa-miR-584-3p 222-229 -0.365 98.0 -0.365 98.0 noncon
hsa-miR-633 548-555 -0.36 98.0 -0.36 98.0 noncon
hsa-miR-1273g-5p 383-390 -0.356 98.0 -0.356 98.0 noncon
hsa-miR-549a 409-416 -0.348 96.0 -0.348 97.0 noncon
hsa-miR-6071 211-218 -0.344 98.0 -0.344 98.0 noncon
hsa-miR-873-5p.2 40-46 -0.343 98.0 -0.343 98.0 noncon
hsa-miR-4330 176-183 -0.334 98.0 -0.334 99.0 noncon
hsa-miR-6880-5p 96-102 -0.331 95.0 -0.331 96.0 noncon
hsa-miR-93-3p 210-217 -0.331 98.0 -0.331 98.0 noncon
hsa-miR-3529-5p 626-632 -0.319 96.0 -0.008 36.0 noncon
hsa-miR-1256 321-327 -0.313 96.0 -0.313 97.0 noncon
hsa-miR-6165 41-47 -0.313 98.0 -0.313 98.0 noncon
hsa-miR-4524b-5p 475-481 -0.312 94.0 -0.312 96.0 noncon
hsa-miR-548an 324-331 -0.307 98.0 -0.307 98.0 noncon
hsa-miR-6868-3p 218-225 -0.307 97.0 -0.307 97.0 noncon
hsa-miR-5579-5p 65-71 -0.3 96.0 -0.3 97.0 noncon
hsa-miR-5100 175-181 -0.287 97.0 -0.287 98.0 noncon
hsa-miR-30d-5p 535-542 -0.282 94.0 -0.282 96.0 con
hsa-miR-30a-5p 535-542 -0.282 94.0 -0.282 96.0 con
hsa-miR-4451 626-632 -0.282 91.0 -0.007 29.0 noncon
hsa-let-7c-3p 608-614 -0.281 97.0 -0.281 98.0 noncon
hsa-miR-9-5p 169-176 -0.281 97.0 -0.281 98.0 noncon
hsa-miR-154-5p 303-310 -0.279 97.0 -0.279 98.0 noncon
hsa-miR-361-5p 299-306 -0.278 96.0 -0.278 97.0 noncon
hsa-miR-4468 571-577 -0.277 95.0 -0.277 97.0 noncon
hsa-miR-1250-3p 556-563 -0.272 99.0 -0.272 99.0 noncon
hsa-miR-1248 217-224 -0.271 96.0 -0.271 97.0 noncon
hsa-miR-487a-3p 589-595 -0.27 97.0 -0.27 98.0 noncon
hsa-miR-154-3p 589-595 -0.27 97.0 -0.27 98.0 noncon
hsa-miR-30e-5p 535-542 -0.266 93.0 -0.266 95.0 con
hsa-miR-4484 325-331 -0.259 98.0 -0.259 98.0 noncon
hsa-miR-4516 93-100 -0.259 98.0 -0.259 98.0 noncon
hsa-miR-5680 622-629 -0.258 99.0 -0.007 67.0 noncon
hsa-miR-379-5p 626-632 -0.258 93.0 -0.007 36.0 noncon
hsa-miR-4524a-5p 475-481 -0.257 90.0 -0.257 94.0 noncon
hsa-miR-628-5p 209-215 -0.252 95.0 -0.252 96.0 noncon
hsa-miR-30b-5p 535-542 -0.25 92.0 -0.25 94.0 con
hsa-miR-30c-5p 535-542 -0.25 92.0 -0.25 94.0 con