EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
DAD1



General Information
Gene Symbol:   
DAD1
Protein name:   
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Enzyme Class:   
CAZy Family:   
E.C. No:   
3.1.1.32
Organs:   
Reaction (SNFG):   

No reaction →
Reaction (IUBMB):   
No specified reaction.
About

• A multi-membrane protein ER membrane associated protein complex that mediates the en-bloc transfer of 14 sugar LLO to N-X-S/T motif in nascent polypeptide
• STT3 is the catalytic core of this complex
• Stt3 forms a complex with the accessory membrane proteins DDOST/OST48, RPN1/ribophorin I, RPN2/ribophorin II, DAD1, Ost4, TUSC3/N33 and MGAT1/IAP in mammals for maximal ativity.
• There are two STT3 genes: STT3A and STT3B. Human Stt3A and Stt3B have redundant functions in the co-translational N-glycosylation. Stt3B also mediates the post-translational N-glycosylation after some time, rather than just at the time when the protein enters the ER from the ribosome.
• Glc[0-3]Man[0-9]GlcNAc2β1-PP-Dol + Asn-Xaa-Ser/Thr -> Dol-PP + Glc[0-3]Man[0-9]GlcNAc2β1-Asn-Xaa-Ser/Thr.

Related Pathway
KEGG ORTHOLOGY:   
K12668
KEGG Pathway:   
ko00510  ko00513  ko01100  ko04141 
Reactome:   
R-HSA-446203
Related Database
Organism:   
Homo sapiens
Uniprot:   
P61803 
NCBI GeneID:   
1603
GlyMap GeneID:   
1603
DNA aRefSeq:   
NM_001344.3 
Protein RefSeq:   
NP_001335.1 
BRENDA:   
Kinetic info.
OMIM number:   
600243
GeneCards database:   
DAD1
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     No reaction
Product:  
     
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 120 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 600 500 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4802-5p 272-279 -0.855 99.0 -0.833 99.0 noncon
hsa-miR-765 270-277 -0.854 99.0 -0.837 99.0 noncon
hsa-miR-3622b-5p 262-269 -0.827 99.0 -0.811 99.0 noncon
hsa-miR-483-3p.1 22-29 -0.798 99.0 -0.798 99.0 con
hsa-miR-378g 157-164 -0.756 99.0 -0.754 99.0 noncon
hsa-miR-6730-3p 174-181 -0.734 99.0 -0.73 99.0 noncon
hsa-miR-1976 147-154 -0.706 99.0 -0.704 99.0 noncon
hsa-miR-4474-5p 31-38 -0.688 99.0 -0.688 99.0 noncon
hsa-miR-519b-3p 119-126 -0.662 99.0 -0.661 99.0 noncon
hsa-miR-519c-3p 119-126 -0.662 99.0 -0.661 99.0 noncon
hsa-miR-519a-3p 119-126 -0.662 99.0 -0.661 99.0 noncon
hsa-miR-4680-5p 162-169 -0.591 99.0 -0.59 99.0 noncon
hsa-miR-22-3p 84-90 -0.551 99.0 -0.551 99.0 noncon
hsa-miR-8068 192-199 -0.545 99.0 -0.54 99.0 noncon
hsa-miR-605-3p 241-247 -0.448 98.0 -0.443 99.0 noncon
hsa-miR-1287-5p 207-213 -0.447 99.0 -0.443 99.0 noncon
hsa-miR-326 159-165 -0.427 99.0 -0.426 99.0 noncon
hsa-miR-3652 208-214 -0.419 99.0 -0.415 99.0 noncon
hsa-miR-4433b-3p 268-274 -0.415 99.0 -0.408 99.0 noncon
hsa-miR-6514-5p 259-265 -0.411 99.0 -0.404 99.0 noncon
hsa-miR-6884-5p 210-216 -0.41 98.0 -0.406 99.0 noncon
hsa-miR-330-5p 159-165 -0.408 98.0 -0.407 99.0 noncon
hsa-miR-4430 208-214 -0.401 99.0 -0.397 99.0 noncon
hsa-miR-216a-3p 168-174 -0.4 99.0 -0.398 99.0 noncon
hsa-miR-485-5p 210-216 -0.399 98.0 -0.395 99.0 noncon
hsa-miR-621 128-134 -0.396 96.0 -0.395 97.0 noncon
hsa-miR-130b-3p 118-124 -0.395 98.0 -0.394 99.0 noncon
hsa-miR-130a-3p 118-124 -0.384 98.0 -0.383 99.0 noncon
hsa-miR-3666 118-124 -0.384 98.0 -0.383 99.0 noncon
hsa-miR-5586-3p 47-53 -0.376 99.0 -0.376 99.0 noncon
hsa-miR-2467-3p 213-219 -0.372 98.0 -0.368 98.0 noncon
hsa-miR-454-3p 118-124 -0.365 98.0 -0.364 99.0 noncon
hsa-miR-6511a-5p 215-221 -0.365 99.0 -0.361 99.0 noncon
hsa-miR-514a-5p 160-166 -0.362 99.0 -0.361 99.0 noncon
hsa-miR-19b-3p 117-123 -0.353 96.0 -0.352 97.0 noncon
hsa-miR-19a-3p 117-123 -0.353 96.0 -0.352 97.0 noncon
hsa-miR-766-5p 271-277 -0.352 98.0 -0.346 98.0 noncon
hsa-miR-27a-5p 156-162 -0.351 97.0 -0.35 98.0 noncon
hsa-miR-301b-3p 118-124 -0.351 98.0 -0.35 98.0 noncon
hsa-miR-301a-3p 118-124 -0.351 98.0 -0.35 98.0 noncon
hsa-miR-1910-3p 215-221 -0.35 99.0 -0.346 99.0 noncon
hsa-miR-5584-3p 255-261 -0.348 99.0 -0.342 99.0 noncon
hsa-miR-4669 95-101 -0.345 99.0 -0.345 99.0 noncon
hsa-miR-6839-5p 206-212 -0.342 98.0 -0.339 98.0 noncon
hsa-miR-3714 216-222 -0.342 99.0 -0.338 99.0 noncon
hsa-miR-4295 118-124 -0.34 98.0 -0.34 98.0 noncon
hsa-miR-6860 158-164 -0.34 98.0 -0.339 99.0 noncon
hsa-miR-4653-3p 258-264 -0.339 98.0 -0.333 98.0 noncon
hsa-miR-3187-5p 158-164 -0.336 98.0 -0.335 98.0 noncon
hsa-miR-488-3p 219-225 -0.335 98.0 -0.331 99.0 noncon
hsa-miR-8076 274-280 -0.328 98.0 0.0 0.0 noncon
hsa-miR-128-3p 168-174 -0.323 98.0 -0.322 98.0 noncon
hsa-miR-6894-5p 271-277 -0.322 98.0 -0.317 98.0 noncon
hsa-miR-612 158-164 -0.321 99.0 -0.32 99.0 noncon
hsa-miR-4680-5p 70-76 -0.318 97.0 -0.318 98.0 noncon
hsa-miR-1285-3p 158-164 -0.315 98.0 -0.314 98.0 noncon
hsa-miR-3681-3p 168-174 -0.313 98.0 -0.312 98.0 noncon
hsa-miR-378j 206-212 -0.31 97.0 -0.307 98.0 noncon
hsa-miR-4486 158-164 -0.303 99.0 -0.302 99.0 noncon
hsa-miR-7154-3p 271-277 -0.303 97.0 -0.298 97.0 noncon
hsa-miR-548an 217-223 -0.3 97.0 -0.297 98.0 noncon
hsa-miR-5189-5p 158-164 -0.291 98.0 -0.29 98.0 noncon
hsa-miR-4533 243-249 -0.286 97.0 -0.283 98.0 noncon
hsa-miR-665 61-67 -0.285 97.0 -0.285 97.0 noncon
hsa-miR-4519 126-132 -0.28 96.0 -0.28 97.0 noncon
hsa-miR-3657 95-101 -0.277 92.0 -0.277 95.0 noncon
hsa-miR-4256 176-182 -0.276 94.0 -0.274 96.0 noncon
hsa-miR-4769-3p 82-88 -0.276 97.0 -0.276 98.0 noncon
hsa-miR-6730-5p 219-225 -0.27 97.0 -0.267 98.0 noncon
hsa-miR-543 41-47 -0.27 99.0 -0.27 99.0 noncon
hsa-miR-548f-5p 237-243 -0.266 98.0 -0.263 99.0 noncon
hsa-miR-6817-5p 82-88 -0.265 97.0 -0.265 98.0 noncon
hsa-miR-3202 243-249 -0.263 94.0 -0.26 95.0 noncon
hsa-miR-320e 86-92 -0.263 96.0 -0.263 97.0 noncon
hsa-miR-6719-3p 175-181 -0.261 96.0 -0.26 97.0 noncon
hsa-miR-6514-5p 273-279 -0.26 96.0 -0.255 97.0 noncon
hsa-miR-3925-3p 76-82 -0.255 94.0 -0.255 96.0 noncon
hsa-miR-4274 126-132 -0.251 95.0 -0.251 97.0 noncon